FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC382394.41837490738453No Hit
TTTGAGGAGCGAGAAGCAGAGACACGAAGATCGGAAGAGCGTCGTGTAGG150.37046184243022967Illumina Single End PCR Primer 1 (100% over 23bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC130.3210669301061991No Hit
TTTGAGGAGCGAGAGGCAGAGACACGAAGATCGGAAGAGCGTCGTGTAGG110.27167201778216843Illumina Single End PCR Primer 1 (100% over 23bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCC100.24697456162015313No Hit
TTTTAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC80.19757964929612248No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGTGATTCACCACTTTTCCC70.1728821931341072No Hit
TTTGAGGAGCTAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC60.14818473697209186No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC60.14818473697209186No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTTGGTGGGATTCACCACTTTTCCC50.12348728081007657No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTTCC50.12348728081007657No Hit
TTTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC50.12348728081007657No Hit
TTTGAGGAGCGATAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC50.12348728081007657No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCAC50.12348728081007657No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCA50.12348728081007657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)