FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA245495.2639751552795No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCCA110.42701863354037267No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCCA80.3105590062111801No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACCA60.2329192546583851No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCCA50.19409937888198758No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCCA50.19409937888198758No Hit
TTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA40.15527950310559005No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTTGGATTCACCACTTTTCCCA40.15527950310559005No Hit
TTGAGGAGCTAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA30.11645962732919254No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCACA30.11645962732919254No Hit
TTGAGGAGCGAGAAGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTAG30.11645962732919254Illumina Single End PCR Primer 1 (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)