FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n01_LL75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n01_LL75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGA161065.98360655737704No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCCATAAGGCTGTGCTG1676.8442622950819665No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACAAGA1355.532786885245901No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTGA612.5No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACAAGA552.2540983606557377No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGACCATCGA451.8442622950819672No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACGAGA391.5983606557377048No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACGAGA220.9016393442622952No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAATAAGGCTGTGCTG200.819672131147541No Hit
ATCGTGTCTCTGCTTCTCGCTCCTCAAAAGATCGGAAGAGCACACGTCTG150.6147540983606558Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAAGGCTGTGCTGACC100.4098360655737705No Hit
ATCGTGTCTCTGCCTCTCGCTCCTCAAAAGATCGGAAGAGCACACGTCTG90.36885245901639346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTTA80.32786885245901637No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCAGTGCATAAGGCTGTGCTGA60.24590163934426232No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGATAAGGCTGTGCTGACCA60.24590163934426232No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGATAAGGCTGTGCTGAC60.24590163934426232No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTATAAGGCTGTGCTG60.24590163934426232No Hit
ATCGTGTCTCTGCTTCTCGCTCCTCAAACGATCGGAAGAGCACACGTCTG50.20491803278688525Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCATAAGGCTGTGCTGACCAT50.20491803278688525No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATAAGGCTGT40.16393442622950818No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGGCCATCGACAAGA40.16393442622950818No Hit
ATCGTGTCTATGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGA40.16393442622950818No Hit
ATCATGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGA30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGAGACAAGAAAGGGACTGAAGC30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGGAGGCTGTGCTGACCA30.12295081967213116No Hit
ATAGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGA30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAGGCTGTGCTGACCA30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGTGCTGACCATCGACGA30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCATAAGGCTGTGCTGACCATCGACA30.12295081967213116No Hit
ATCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTATAAGGCTGTGCTGACC30.12295081967213116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)