FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n01_LL21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n01_LL21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTGACC186441.34871339840284No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGACC97621.650399290150844No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCCATAAGGCTGTGCTGAC59913.287488908606921No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACGAGAAA821.8189884649511978No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACGAGAAA741.6415261756876662No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACAAGAAA651.4418811002661935No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACAAGAAA591.3087843833185449No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGACCATCGACG541.1978704525288377No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGATAAGGCTGTGCTGACCA340.7542147293700089No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAATAAGGCTGTGCTGAC300.6654835847382431No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTATAAGGCTGTGCTGAC290.6433007985803016No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTATAAGGCTGTGCTGACCAT210.4658385093167702No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAAGGCTGTGCTGACCAT180.39929015084294583No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTTACC140.3105590062111801No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTCATAAGGCTGTGCTGACCA140.3105590062111801No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGATAAGGCTGTGCTGACCATC140.3105590062111801No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTAAGGCTGTGCTGACCA120.26619343389529726No Hit
CGTGTCTCTGCTTCTCGCTCCTCAAAAGATCGGAAGAGCACACGTCTGAA110.2440106477373558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGAATAAAGCTGTGCTGACC100.22182786157941436No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTTACC90.19964507542147292No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGACCATCGACA90.19964507542147292No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCATAAGGCTGTGCTGACCATCG70.15527950310559005No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGGCTGTGCTGAC60.13309671694764863No Hit
CGTGTCTCTGCTTCTCTCCCCTCCATAAGGCTGTGCTGACCATCGACGAG60.13309671694764863No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAAATAAGGCTGTGCTGA60.13309671694764863No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTTAAAGCTGTGCTGACC60.13309671694764863No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGTGCTGACCATCGACGAGA60.13309671694764863No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGGCTGTGCTGACCATCGAC50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCGAATAAGGCTGTGCTGA50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCCATAAGGCTGTGCTGACCATCGACAAG50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCATAAGGCTGTGCTGACCATCGACAAGA50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCGATAAGGCTGTGCTGAC50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGTGCTGACCAT50.11091393078970718No Hit
CGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGTGCTGACCATCGACAAGA50.11091393078970718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)