FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n01_LL18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n01_LL18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6568
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTGAC269140.97137637028014No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTGAC125619.12302070645554No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCCATAAGGCTGTGCTGA72711.068818514007308No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACAAGAA2353.5779537149817298No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGACCATCGACGAGAA1772.694884287454324No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACAAGAA1712.6035322777101095No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGACCATCGAC1231.8727161997563948No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCTGTGCTGACCATCGACGAGAA1091.6595615103532277No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAATAAGGCTGTGCTGA711.0809987819732034No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTATAAGGCTGTGCTGA420.6394640682095006No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGATAAGGCTGTGCTGACC270.4110840438489647No Hit
TCGTGTCTCTGCTTCTCGCTCCTCAAAAGATCGGAAGAGCACACGTCTGA260.3958587088915956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAAGGCTGTGCTGACCA220.33495736906211937No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTAAC160.24360535931790497No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCATAAGGCTGTGCTGACCATC150.22838002436053592No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCTTAC130.1979293544457978No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTAAGGCTGTGCTGACC110.16747868453105969No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGATAAGGCTGTGCTGACCAT100.1522533495736906No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATATAAGGCTGTGCTG100.1522533495736906No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCAAAGGCTGTGCTGACC90.13702801461632155No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTGTGCTGTGCTGACCA90.13702801461632155No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCCATAAAGCTGTGCTGA90.13702801461632155No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTCATAAGGCTGTGCTGACC80.12180267965895249No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGTGCTGACCATCGACGAG80.12180267965895249No Hit
TCGTGTCTCTGCCTCTCGCTCCTCAAAAGATCGGAAGAGCACACGTCTGA80.12180267965895249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAGGCTGTGCTTAC70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGTGCTGACCATCGACAAG70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGGCTGTGCTGA70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCCATAAGGCTGTGCTGACCAT70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCATAAAGCTGTGCATAA70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGGCCGTGCTTACCATCGACGAGAA70.10657734470158343No Hit
TCGTGTCTCTGCTTCTCTCCCCTCCAGATAAGGCTGTGCTGACCATCGAC70.10657734470158343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)