FastQCFastQC Report
Sat 14 Sep 2024
000000000-DR2YL_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DR2YL_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517826
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC15255229.46008891017446Illumina Single End PCR Primer 1 (100% over 8bp)
GTCAGATA238024.5965247013475565No Hit
TCGACGAG233524.509622923530298No Hit
CGTTACTA225324.35126857284107No Hit
GATCGTGT218214.213963763889801No Hit
TACGAGAC217154.19349356733729TruSeq Adapter, Index 14 (100% over 8bp)
ACGTCTCG213584.124551490268932No Hit
CTACTATA203443.928732817587375No Hit
AGAGTCAC180773.490940972450205No Hit
GACACCGT143522.7715873671851163No Hit
CTGCGTGT139592.695693147891377No Hit
TAGCGAGT135362.614005476743153No Hit
TCATCGAG132342.5556847280746817No Hit
CGTGAGTG127222.456809816424822No Hit
GGATATCT112942.1810415081513868No Hit
ACTATCTG104322.0145763248658817No Hit
ATCGTACG102581.9809743041098746RNA PCR Primer, Index 22 (100% over 8bp)
TAGTGTAG79521.535650971561876No Hit
GCTCTAGT74331.4354242544793039No Hit
CGTCGCTA71731.3852143384071096No Hit
GACACTGA71371.378262196181729No Hit
TGCGTACG63351.2233839166051916No Hit
CTAGAGCT60601.1702772746057557No Hit
ATATACAC44080.8512511924855068No Hit
ACGACGTG33380.6446180763422462No Hit
CCGTCTCG5220.10080606226802052TruSeq Adapter, Index 14 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)