FastQCFastQC Report
Sat 14 Sep 2024
000000000-DR2YL_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DR2YL_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517826
Sequences flagged as poor quality0
Sequence length8
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTACC185703.586146697925558No Hit
CGAAGTAT153072.9560122512195215No Hit
GATCTACG148082.859647835373272No Hit
TAGCAGCT147392.846322896107959No Hit
CTCGACTT141912.7404958422327192No Hit
GCGTATAC125002.413938272701641No Hit
TCTCTATG124242.399261528003615ABI Solid3 Adapter B (100% over 8bp)
ACGTGCGC118212.282813145728488No Hit
ATGAGCTC106712.060730824639937No Hit
TGCTCGTA103652.0016376157242006No Hit
CGAGCTAG101601.9620490280518939No Hit
ATAGCGCT101211.9545175406410646No Hit
GTAACGAG100231.9355922645830839No Hit
CTCTAGAG98251.89735548234349No Hit
TCTAGACT95771.8494629470130894No Hit
TCCTCATG94431.8235855287297278No Hit
TCAGTCTA93731.8100674744025986No Hit
AGCATACC90571.7490431148687011No Hit
CGTCATAC90111.7401598220251593No Hit
CATCGTGA64521.245978378837679No Hit
AGTCGCAG59001.1393788647151746No Hit
CAGTAGGT57441.1092529150718582No Hit
CTATCGTG50020.9659615392042887No Hit
TACTAGGT49810.9619061229061499No Hit
TAGTCTCC48900.944332652280882No Hit
ACGTACGT47580.9188414641211528No Hit
AACGCTGA47300.913434242390301No Hit
CGCGATAT45610.8807977969433748No Hit
ACTCACTG45520.8790597613870296No Hit
GTTACAGC45410.8769354957070522No Hit
TAACGTCC45010.8692108932344069No Hit
CTACGACC43990.8495131569291615No Hit
CGAGAGTT42210.8151386759258903TruSeq Adapter, Index 13 (100% over 8bp)
ACGCTACT41200.795634054682461No Hit
GCGATACG41080.7933166739406673No Hit
ACTACGAC40710.7861714166534705No Hit
CGAGCGAC38350.7405962620648635No Hit
GACATAGT38280.7392444566321505No Hit
CTGCGTAG37820.7303611637886085No Hit
GAGACTTA37360.7214778709450664No Hit
TGAGTACG36770.7100840822979148No Hit
AAAAAAAA35670.6888414254981403No Hit
CGTAGCGA35590.6872965050036113No Hit
GTCTGCTA33780.6523426788148915No Hit
ACGTATAC17240.3329303665710103No Hit
ACTGTGTA14220.2746096179025387No Hit
GAAAAAAA12690.2450630134446706No Hit
AATCTACG10900.2104954173795831No Hit
AAAAATAA10390.20064654922696043No Hit
AAAATAAA10100.1950462124342926No Hit
TTTTTTTT10000.19311506181613128No Hit
AAAAAATA9920.19157014132160222No Hit
ATAACGAG9220.17805208699447306No Hit
AATAAAAA8070.15584385488561794No Hit
TAAAAAAA7900.15256089883474372No Hit
AAATAAAA7530.14541564154754685No Hit
AAAAAAGA6750.1303526667258886No Hit
AAAAAAAT6400.12359363956232403No Hit
AAAAGAAA5300.10235098276254959No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)