FastQCFastQC Report
Thu 1 Aug 2024
000000000-DPHLP_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DPHLP_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951266
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC23213524.402743291571444Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAATGA190582.003435421848358No Hit
CGCGGTTA178731.8788645867717335No Hit
TGGCCATG176091.851112096931878No Hit
GAGAGATT167841.7643855661823298No Hit
CACGTCAG166021.7452531678836414No Hit
ATTGACAT161971.7026783255156812RNA PCR Primer, Index 15 (100% over 8bp)
TAAGATGG158061.6615752060937738No Hit
TCCTTACG157281.6533756068229075No Hit
ACGCGTGG156031.6402352233760062No Hit
TCACGGTT153371.6122724874010004No Hit
GACCGCCA150971.5870429511829498No Hit
AGCCAACT140521.477189345566855No Hit
TACTAGGT129481.361133478963823No Hit
TCGTGGAA129261.3588207714771683No Hit
CGGAAGAA121981.2822911782824151No Hit
AGCACCAA120471.2664175950785586No Hit
TCCAACAG114311.201661785452229No Hit
AGGAACTG107451.1295473610956346No Hit
ATGGATGC105901.1132532856214772No Hit
GCACTAAC105361.1075766399724158No Hit
CCTCAGAC104691.1005333944448767No Hit
TGAGGCAT104641.1000077791070004No Hit
GGAACTCC103851.0917030567685588RNA PCR Primer, Index 1 (100% over 8bp)
ATGTCGTG103561.0886544878088777No Hit
ACTGCATA102891.0816112422813389No Hit
CACTGAGA102801.080665134673162No Hit
AAGGTACC100111.0523870294954303No Hit
AAGGAGTA98761.0381954153727768ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
CTCTCTAT98271.0330443850615916ABI Solid3 Adapter B (100% over 8bp)
TCGGTTCA91830.9653451295431561No Hit
TTGTGAGC91760.9646092680701298No Hit
TATCCTCT87110.9157270416476568No Hit
AGACGGAA86920.9137297033637279TruSeq Adapter, Index 14 (100% over 8bp)
TTGCCTGA86460.9088940422552683No Hit
GACAATGC82030.86232452331945No Hit
AGTTCAGG79210.8326798182632408No Hit
TGTAGGTG79170.8322593259929398No Hit
AGCATTGG79090.8314183414523382No Hit
CGTGAGTG78610.8263724342087282No Hit
ATAGCCTC77180.8113398355454731No Hit
GCAGAGAA74670.7849539455840953No Hit
TGCGTGAA74660.7848488225165201No Hit
GGATATCT74580.7840078379759184No Hit
GGCTACTC71640.7531016561088065No Hit
AGAGTAGA68890.7241928125256237No Hit
TGTTAGCC68030.7151522287141556No Hit
GTAAGGAG65960.6933917537260872No Hit
CTAAGCCT64940.6826692008334156No Hit
GTGCTGAA64460.6776232935898057No Hit
TAGATCGC63480.667321232967435No Hit
ATACGTCC62940.6616445873183736No Hit
TCATCGAG62270.6546013417908345No Hit
CCGTAACA57660.6061396076386626No Hit
ACTATCTG56210.590896762840257No Hit
TCTCTGGA54330.5711336261361176No Hit
CATAGACC52950.5566266428107386No Hit
ATCGTACG52800.5550497967971104RNA PCR Primer, Index 22 (100% over 8bp)
AGCTAGAG52710.5541036891889335No Hit
GACACCGT52580.5527370893104557No Hit
TAGCGAGT49460.5199386922269902No Hit
GGCCATAA49180.5169952463348843No Hit
AGGTTGCA48820.5132108159021767No Hit
CGGTTAAC48530.5101622469424956No Hit
CTGCGTGT46050.4840917261838434No Hit
CTAGGAAC45690.48030729575113584No Hit
AGTCCTTC45470.4779945882644812No Hit
CTGTGCTA40350.4241715776659736No Hit
TTACCGAC36120.3797045200816596No Hit
TCGAAGTC34970.3676153673105104No Hit
CAATGGTC33640.35363399932300743No Hit
CTGACTTC27900.29329335853483673No Hit
GTGAACCA24210.2545029465995841No Hit
TCGACGAG16410.1725069538909201No Hit
CGTTACTA13540.14233663349683476No Hit
GTCAGATA12500.13140383446901285No Hit
AGAGTCAC11910.12520157348207545No Hit
TCTACACT11830.12436058894147378Illumina Single End PCR Primer 1 (100% over 8bp)
CTACTATA11340.11920955863028847No Hit
GATCGTGT10360.10890749800791787No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)