FastQCFastQC Report
Thu 1 Aug 2024
000000000-DPHLP_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DPHLP_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951266
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATGC296153.113219646239853No Hit
ACAGATTC269652.8346435171655457No Hit
ATCATTCC222922.343403422386588No Hit
ACACGACC218112.292839226882912No Hit
AATCCGTC217002.2811705663820634No Hit
AGCACCTC214472.254574430285535No Hit
CCTAAGAC208642.193287681889188No Hit
CGAGGCTG205232.157440715846041No Hit
TGGCTTCA198662.088374860449128No Hit
GCTACGCT196482.065458031717732No Hit
TCTTCACA194152.040964356972708No Hit
ACTGAGCG183381.9277468131942064No Hit
AAGGACAC178351.8748699102038757No Hit
TTCACGCA173931.8284055143356328No Hit
CGATCAGT166301.7481966137757474No Hit
TATCAGCA159411.6757668202164275No Hit
ACTCGCTA158511.6663057441346583No Hit
CAGAGAGG157381.6544268374986597No Hit
GCTCATGA147541.5509857390046529No Hit
CTCTCTAC146641.5415246629228838No Hit
TCCGTCTA145581.5303816177599117No Hit
TAGCGCTC134481.413695012751428No Hit
ATCTCAGG112701.1847369715726201No Hit
ATGCGCAG97191.021691093763469No Hit
CTCGTTGT96561.0150683405062306No Hit
GCGTATAC95080.9995101265050996No Hit
TAGGCATG95010.998774265032073No Hit
GGACTCCT94680.9953052038020912No Hit
GTAACGAG94240.9906797888287818No Hit
AAGAGGCA93300.9807982204767122No Hit
CGGAGCCT91510.9619811913807494No Hit
CTCGACTT89160.937277270500575No Hit
CGAAGTAT86990.9144655648367543No Hit
TACGCTGC84820.8916538591729337No Hit
GCGTAGTA84510.8883950440781022No Hit
GGAGCTAC80120.8422460174125849No Hit
TGCTCGTA76830.8076605281803407No Hit
CAACAGAG74860.7869512838680244No Hit
AGGCAGAA73960.7774902077862553No Hit
CGTACTAG71770.7544682559872843No Hit
CTAATGGC70640.7425893493512856No Hit
TCCTGAGC69470.7302899504449859No Hit
CACCTATC68390.7189366591468632No Hit
CCTTGAAG67820.7129446442950762No Hit
GATCTACG64890.6821435854955397No Hit
TTGGAGAG62030.6520783881690295No Hit
ACCAAGGA61890.6506066652229765No Hit
GTCTTGTC61040.6416712044790837No Hit
TAAGGCGA54220.5699772723927903No Hit
GCCATACA52010.5467450744586688No Hit
GAATCCAG51970.546324582188368No Hit
GGTAACGT48320.5079546625234161No Hit
GTAGAGGA44970.4727384348857207No Hit
TCATTGCG37400.39316027273128656No Hit
CTACGACC16910.17776310726968061No Hit
TAACGTCC15220.15999730884947008No Hit
GTTACAGC12630.1327704343474906No Hit
ACGTGCGC10650.11195606696759897No Hit
ACGTACGT10460.10995872868366997No Hit
GCGACTAA9940.10449232916975905No Hit
ATAGTACC9850.10354622156158215No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)