FastQCFastQC Report
Thu 18 Jul 2024
000000000-DPHKV_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DPHKV_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782538
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC19317724.685957742627195Illumina Single End PCR Primer 1 (100% over 8bp)
TGCGTGAA200982.5683097817613967No Hit
TGAGGCAT183812.348895516895026No Hit
GAGAGATT178742.2841063309385614No Hit
CATAGACC153071.9560711428710174No Hit
CGGTTAAC152191.9448256825866606No Hit
AGTTCAGG148421.8966491084139045No Hit
TCCTTACG146601.873391451916712No Hit
CTAGAGCT143261.8307098185647215No Hit
TGGCCATG136061.7387015071472567No Hit
TAGTGTAG134571.719660898256698No Hit
TGCGTACG134521.7190219516496326No Hit
CGTCGCTA127541.6298250053032568No Hit
ACGACGTG115971.4819727604282475No Hit
GGAACTCC115971.4819727604282475RNA PCR Primer, Index 1 (100% over 8bp)
AGCACCAA114641.4649767806802991No Hit
AGGAACTG113981.4565426854670316No Hit
TGTAGGTG113921.4557759495385527No Hit
ATATACAC108811.3904756062964354No Hit
TGTTAGCC101441.2962948764149473No Hit
GCTCTAGT99491.2713759587393838No Hit
CCTCAGAC93221.1912520542133418No Hit
GACACTGA89971.1497205247540694No Hit
ATGTCGTG89801.147548106290046No Hit
GACAATGC87931.1236515031857879No Hit
TATCCTCT82851.05873452790791No Hit
ACGCACAA81831.045700017123769No Hit
CGCGGTTA81691.043910966623985No Hit
GTGATAGG76490.9774605194891494No Hit
CTCTCTAT75200.9609756970268536ABI Solid3 Adapter B (100% over 8bp)
GTTCGAGA74370.9503691833495627No Hit
ATACGTCC73280.9364401473155297No Hit
CTAGGAAC73160.934906675458572No Hit
ACGCGTGG71230.9102433364258349No Hit
ACTGCATA68130.8706286467877599No Hit
GTCACAAC68060.8697341215378678No Hit
GTAAGGAG66200.8459653077550228No Hit
AGCATTGG66200.8459653077550228No Hit
CCGTAACA66160.8454541504693701No Hit
GCACTAAC64280.8214297580436989No Hit
TTGCCTGA63590.8126122948661918No Hit
CGGAAGAA63560.8122289269019524No Hit
TAGATCGC63350.8095453511522762No Hit
CTTGAGGA62890.8036670423672716No Hit
GCAGAGAA62420.7976609442608538No Hit
TTACCGAC62380.7971497869752012No Hit
ATAGCCTC61450.7852653800837786No Hit
AGAGTAGA60600.7744032877636613No Hit
TCACGGTT59630.7620077235865862No Hit
TCGGTTCA57850.7392612243750463No Hit
AACTCTCG57600.7360664913397177TruSeq Adapter, Index 13 (100% over 8bp)
CTGACTTC54430.6955572764517506No Hit
CACGTCAG51660.6601596344203092No Hit
TGTCGAAC51060.6524922751355205No Hit
GTTAGCTC49780.636135241994638No Hit
TAAGATGG49180.6284678827098492No Hit
CTAAGCCT49080.6271899894957178No Hit
TGTAATGA47930.6124942175332061No Hit
AAGGAGTA44820.5727517385737178ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
AGCCAACT38310.48956089033376016No Hit
AGACGGAA35530.45403545898090575TruSeq Adapter, Index 14 (100% over 8bp)
GGCTACTC34820.4449624171605724No Hit
CTGTGCTA31920.40790351395076024No Hit
ATTGACAT31880.4073923566651076RNA PCR Primer, Index 15 (100% over 8bp)
GTGAACCA26820.34273096003005604No Hit
TCTCTGGA26010.33238002499559127No Hit
TACTAGGT23460.29979374803523917No Hit
GTGCTGAA23040.2944265965358871No Hit
AGCTAGAG19360.24740012625584956No Hit
AGGTTGCA18100.23129867175779323No Hit
GACCGCCA16240.20752985797494816No Hit
AGTCCTTC14810.1892559850128684No Hit
GGCCATAA12990.16599832851567592No Hit
CACTGAGA9080.11603270384313606No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)