FastQCFastQC Report
Thu 18 Jul 2024
000000000-DPHKV_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DPHKV_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782538
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGAGCG318104.064978314152156No Hit
CCTAAGAC303463.877894747603311No Hit
CGATCAGT294763.7667180379738747No Hit
TAGCGCTC291123.72020272497949No Hit
ATGCGCAG197042.5179607891246176No Hit
TACGCTGC177042.262382146298327No Hit
ACTCGCTA174592.231073762552106No Hit
ATCTCAGG170312.17637993298728No Hit
TCCGTCTA159402.036961783325538No Hit
TCTTCACA159302.035683890111407No Hit
AAGGACAC154321.9720448080476602No Hit
CGGAGCCT150431.922334762017947No Hit
TTCACGCA144191.8425942254561438No Hit
GGAGCTAC141521.808474476638834No Hit
GATCTACG131631.6820908377612334No Hit
TGGCTTCA124001.5845875855230034No Hit
ACAGATTC123141.5735977038814728No Hit
AGCACCTC122001.5590297212403743No Hit
GTAACGAG120711.5425448987780785No Hit
AGCCATGC115381.474433190464872No Hit
AATCCGTC108411.3853640334399095No Hit
GCTCATGA107481.373479626548487No Hit
ACACGACC101591.2982117162361444No Hit
TATCAGCA92841.1863960599996421No Hit
GCTACGCT84541.0803309232267315No Hit
GCGTAGTA82061.0486391715162715No Hit
TGCTCGTA73600.9405294056007504No Hit
ACGCTACT68880.8802128458937457No Hit
CGTAGCGA68440.8745901157515673No Hit
TGAGTACG61770.7893546383689992No Hit
AGGCAGAA60800.7769590741919242No Hit
CAGAGAGG59810.7643079313720228No Hit
TAGTCTCC57380.7332551262686284No Hit
ATCATTCC57360.7329995476258022No Hit
ACTCACTG57190.7308271291617787No Hit
CGAGGCTG55130.7045025289506708No Hit
AAGAGGCA54360.6946627512018586No Hit
GACATAGT54150.6919791754521825No Hit
CACTAGCT51920.6634821567770511Illumina PCR Primer Index 10 (100% over 8bp)
AACGCTGA51370.6564537440993281No Hit
CGAGAGTT50740.6484030168502999TruSeq Adapter, Index 13 (100% over 8bp)
CGTACTAG49170.6283400933884361No Hit
CTCTCTAC47860.611599692283314No Hit
TAGGCATG46230.5907700328929714No Hit
GAACCACA43160.5515387112191357No Hit
GTCTGCTA40850.5220193779726992No Hit
CTGCGTAG39880.5096238137956239No Hit
GTAGAGGA39780.5083459205814925No Hit
GGTTGCAA38880.49684488165430946No Hit
GGACTCCT36160.4620861862299339No Hit
ACTACGAC36040.4605527143729761No Hit
TCCTGAGC35430.4527575657667743No Hit
CGAGCGAC35240.4503295686599245No Hit
TAAGGCGA28210.3604936757064833No Hit
GCGACTAA27900.3565322067426757No Hit
GCATAAGG23030.29429880721447393No Hit
ACCGGTAA21930.28024198185902793No Hit
GTCTATGA21090.26950767886032373No Hit
ACAGTGGT19650.2511060165768308No Hit
AACCTCCA19610.2505948592911782No Hit
GATTCGGA18520.23666582325714533No Hit
CATCTTCG18290.233726668864643No Hit
CATTACGG14070.17979957522829562No Hit
CGTGTACA12730.16267580615893412No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)