FastQCFastQC Report
Thu 23 May 2024
000000000-DP893_l01_n02_Kas-2_rep3_flg22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n02_Kas-2_rep3_flg22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACATCCTGCCTGTTGATGATACTGTTGAAGGAGTGACTGGAAACCTAT12.3255813953488373No Hit
TGAGAAGGTTTTGTCATGTGGAGAAACGGAAGATCTGCAACTGAATCTGG12.3255813953488373No Hit
TAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGC12.3255813953488373No Hit
TTCTTTTTTCTCTCTCTTTTTCTTCTTTTCTTTTCTTTCTGCTTTTTTCT12.3255813953488373No Hit
TTTTTTTTTTCTTCTTTTTTCAGTCTTCTTCTTTATTCTTGTACTTTTTT12.3255813953488373No Hit
GGACTGTGTTTGGATCTTCCTATTCTGACAGCAGGGGAAGTAATATAGCA12.3255813953488373No Hit
GGAAACTTTAAGACATTAACATATCTCCCTCTGAGTCTAACGTATCTAGA12.3255813953488373No Hit
TTTTCAACTGCTATTATAGCTATTTCTATTATCTCATATCCATAATATTC12.3255813953488373No Hit
TGCATTCTTCTTCAACTACGTCGACGATGATCATGTGAAAGCAATGCTCG12.3255813953488373No Hit
ACCCAGTTATTGATGGGTCTCAGAGAGTACAACTTATCAACCAACCTGCT12.3255813953488373No Hit
TACGCAAATAGACTAAATCAAATCTCTTTTCGGACACAGGTTACTAACTG12.3255813953488373No Hit
GCCGTTCAAGTTACGGTGCTAAATGAATTTTACCAGATGAAGAATCAGTC12.3255813953488373No Hit
TGTCTCTCATGGGTTCAGTGACCACTAGCAACTTCTTGAATCCTCAGGAT12.3255813953488373No Hit
TTGAGATTTGCCAGAGAGCTTGCAAGTACGCCATCAGAGAAAACATTGAG12.3255813953488373No Hit
TCTTTTCTTTCCAATTCCACCAAGCTCGTGTCTTTGATACTCAACAACTG12.3255813953488373No Hit
TCTTTTTTTGTTGTTTTTCTCTCTTTCTCGTTTTGATTCTTGTGGCTCTC12.3255813953488373No Hit
CTCTGTTGTCTTTGACAGGATTGAGCAATCAAAAATGTCGATTGTCGTCT12.3255813953488373No Hit
CCCAACAGAAGAATCACTGTTGAACAAGCTCTGAATCACCAGTACCTTGC12.3255813953488373No Hit
TTTTTATCAACTTCCTCCCCACAGCCTCCACCAGAATCCAACCAGTTCGG12.3255813953488373No Hit
CCAGCAGTATCTTTTGGGGAAAAGGTACTTGATAGTTATGGACGATGTAT12.3255813953488373No Hit
TTTGTGATTTCGGTCTTGCTAGACCTACTTCAGAGAATGATTTTATGACT12.3255813953488373No Hit
GGAAACTTGAATCTGAACATGTGTTTATCAGTTATAACAACTGTAATGTG12.3255813953488373No Hit
CTTAAGGGGATGCAATGTGAATGAAGACAAAGAAATTCCTGCTCTTTCAC12.3255813953488373No Hit
GTTCCGGTGGATATCTTAAGCTGTAAGCAACTTGAAGTTCTTGATCTTCG12.3255813953488373No Hit
TCTCAATTTCTTCCTTTTCATTAGCCCCGTTTTTGAATCCGTTGTATCCG12.3255813953488373No Hit
TTATTCTCTGCCTGTGGAAATCTGTGAACTGCCAAGACTAAAGTATGTTG12.3255813953488373No Hit
CTTGAAGATAAGTGCTTTGTAACGATTTCTTATAATCGTCTTGAGATGCA12.3255813953488373No Hit
TGCATTTATGTGGGAAGAAGTTGAGAAGGCTGTTCCAGGATTAAAGATCG12.3255813953488373No Hit
TATGTAGAAGATGATGTGAAAGGTGTGATTGGTTTTGTTGATCCGGACTA12.3255813953488373No Hit
GAAAATACCTTGACCATTCCTCTCGAAAGGAAAAGGTCTTACCGTGGCGT12.3255813953488373No Hit
TTTAAATAATGGTCCAATGTCATCCGCTCAGGCACCGAGATATCATTACC12.3255813953488373No Hit
ATCACCAGGTGATCATTTTCAAATCTCGACCATTTTGAGCAAAAATCCCT12.3255813953488373No Hit
TTGAGGCTGAAGCAAGCAGAGGCAGAGATGTTTCAGTTAAAAGCAAACGA12.3255813953488373No Hit
CCCTGACCTCGATAACATGATTATTGAGTGTGGTGTACAGATCATGACGG12.3255813953488373No Hit
AAAAGATTAGGCCTCCAATGGAGAAGAAAAACCAAAGAGCGCAAGGCAGC12.3255813953488373No Hit
CACATAGTGATGATTCCGTGCAAGCCGCTGCTATTCGGATTCAGAACAAG12.3255813953488373No Hit
TTAGTACTCGAATGTGCAACAGGAAAGTTCCCTTATGCACCTCCGAATCA12.3255813953488373No Hit
TTTTTGTGTGTTTTGCTGTTACGCGAGCTCAACGCAAGTTACTTGCTTTT12.3255813953488373No Hit
CTTTCTTTAGCCATAAACCTCGAGGAATTAGATCTTGTTGGATGCGAATC12.3255813953488373No Hit
TGGGAGCCTGATCTTGCTTGATGGGAAACAAGATGTTATTTGGTCAACAG12.3255813953488373No Hit
CATGTGCAGCAATCAAGATGTGCTGAGGAGTTCAAGCTGAGGAAGAAGAA12.3255813953488373No Hit
TCTGAAGAGAACTAATGAAACGTTGCGTACTCTTGTTGCGGATTTACGAG12.3255813953488373No Hit
CTTCGGAACGGGCCTAATGATGCGCATATGGTTGAAAAGATAGCCAATGA12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)