FastQCFastQC Report
Fri 24 May 2024
000000000-DP893_l01_n01_Miseq_Pla-0_rep2_flg22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n01_Miseq_Pla-0_rep2_flg22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21067
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAATTTTCCTTTAAGATGTTTGACCACATCGAGATCAATCCTGTAGA290.1376560497460483No Hit
CCAAATTCCAATCATCTTGACTTCATCACTTTCCAGGCATAACAAAGAGT270.12816252907390707No Hit
GGCATAACAAAGAGTTCAATCTTTTCACGTGAGCTTCCATCCCTACCATC260.12341576873783644No Hit
CCCTTACTTACCGAAACCTCTTCGATATTGGATGTATCATATGATATACC250.11866900840176578No Hit
CCCGAACTCTCCACTCTGTTTCTTAACATCTGACGGATCAACTTCGTAAA230.10917548772962453No Hit
CCCCTCTCCTCTTGTTCGTCTATAAACACGTTGATCTTTTCTTTTTTCAA230.10917548772962453No Hit
CTCATTTTTCCCTTGACAAGTCTTCTCAAAAGCTTCCCCGAACTCTCCAC220.10442872739355391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)