FastQCFastQC Report
Fri 24 May 2024
000000000-DP893_l01_n01_Miseq_Nyl-2_rep3_flg22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n01_Miseq_Nyl-2_rep3_flg22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10209
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATTTCAGATATCTTGGCCTTGATTTCTTTGATATCGTCCCATTGAT260.2546772455676364No Hit
GTCCCATTGATCAACTTGAAAATCCACAACTATTATGCTTCTTAATGCAA200.19590557351356644No Hit
GCCGAATTTTCCTTTAAGATGTTTGACCACATCGAGATCAATCCTGTAGA180.17631501616220982No Hit
CTCCATTTAAGAACTTTCTCAGGATCTTCTCTACTCGCATGTTTCTTAAA150.14692918013517484No Hit
CTCCCATTGCTTATTGTTGTAACAAGCATCAGCTCTTTTGATATCTTGTC130.1273386227838182No Hit
CTTGATTTCTTTGATATCGTCCCATTGATCAACTTGAAAATCCACAACTA130.1273386227838182No Hit
GTCCCATACATCGTCCATAACTATCAAGTACCTTTTCCCCAAAAGATACT120.11754334410813988No Hit
GCACTGGTGACTGATTTTTCATCTGGTAAAATTCATTTAGCACCGTAACT110.10774806543246156No Hit
GACGAATTGTAACACCTTTGACGAAGAGCCCTGACTTCCACAGTTGGCAC110.10774806543246156No Hit
CCCCAAGTTCCGCAATATGCTTCTCATGATCTGTTCTTCAGTAAAAGTCT110.10774806543246156No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT110.10774806543246156No Hit
CCCGACGTTTTTCTTCGATGTGGTGAACTCTCCAACCGGAATATCGAGCC110.10774806543246156No Hit
CCCCGACGTTTTTCTTCGATGTGGTGAACTCTCCAACCGGAATATCGAGC110.10774806543246156No Hit
TCCTCATCCACTTTATCAGACTCATCTCGTCAGTATGCTGAAAAAACTCA110.10774806543246156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)