FastQCFastQC Report
Fri 24 May 2024
000000000-DP893_l01_n01_Miseq_Kas-2_rep3_Mock.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n01_Miseq_Kas-2_rep3_Mock.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12044
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTCTGTGCACAAAGCTGCAAGATCAGCACCGACGTAACCGTGTGTGT190.15775489870474926No Hit
CCACCGTCGTTTTTATTACCATTCACGTACAAAACCAATCTCCATTTGTA150.12454334108269677No Hit
GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC150.12454334108269677No Hit
CTCCTCTCTGAGTTGCAATGGAGTCGAGCTCATCAAAGAAAAGAACACAT140.11624045167718366No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA140.11624045167718366No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT140.11624045167718366No Hit
CCTCTCTCTTCACAGGCTCACCCTCACAGAAAATCTCTGTGTCTGGAGCA130.10793756227167055No Hit
CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGT130.10793756227167055No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG130.10793756227167055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)