FastQCFastQC Report
Fri 24 May 2024
000000000-DP893_l01_n01_Miseq_Kas-2_rep2_Mock.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n01_Miseq_Kas-2_rep2_Mock.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10394
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT160.1539349624783529No Hit
CCCCTCTAGAGATGTTGATGATGATGGTTCTCCCTGCCCTAACGAAAGCT150.14431402732345583No Hit
GGCCTCTGTGCACAAAGCTGCAAGATCAGCACCGACGTAACCGTGTGTGT130.12507215701366173No Hit
CCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC120.11545122185876468No Hit
CCTCCATTGCCTCTGGGTTCTCGCTCCTCCTCTTCTCCTTTTCAATGTCC110.10583028670386763No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA110.10583028670386763No Hit
CCTCTCTCTTCACAGGCTCACCCTCACAGAAAATCTCTGTGTCTGGAGCA110.10583028670386763No Hit
GTTCGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGC110.10583028670386763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)