FastQCFastQC Report
Fri 24 May 2024
000000000-DP893_l01_n01_Miseq_Col-0_rep2_Mock.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DP893_l01_n01_Miseq_Col-0_rep2_Mock.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13675
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTCATTAAAGTTAGTTCTTCCTCCCACGAGATGAGATCCAATATTCT250.18281535648994515No Hit
CCTCTCTCTTCACAGGCTCACCCTCACAGAAAATCTCTGTGTCTGGAGCA220.16087751371115175No Hit
GGCCTCTGTGCACAAAGCTGCAAGATCAGCACCGACGTAACCGTGTGTGT210.15356489945155394No Hit
GTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCT160.11700182815356491No Hit
CTCCCACGAGATGAGATCCAATATTCTCAGATAATCTTCTCCAATCATGC160.11700182815356491No Hit
CCACCGTCGTTTTTATTACCATTCACGTACAAAACCAATCTCCATTTGTA150.10968921389396709No Hit
CTCCTCTCTGAGTTGCAATGGAGTCGAGCTCATCAAAGAAAAGAACACAT140.10237659963436929No Hit
CGTCATTAAAGTTAGTTCTTCCTCCCACGAGATGAGATCCAATATTCTCA140.10237659963436929No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)