FastQCFastQC Report
Fri 19 Jan 2024
000000000-DNCDK_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DNCDK_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711464
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC15828422.247647105124084Illumina Single End PCR Primer 1 (100% over 8bp)
AGTTCAGG204592.875619848650107No Hit
TCCAACAG192092.6999257868282864No Hit
TGTAGGTG182672.5675227418393622No Hit
CACTGAGA181862.556137766633308No Hit
CCGTAACA168662.370604837349465No Hit
TTGCCTGA166972.346851000191155No Hit
ATAGCCTC165582.3273138205165687No Hit
TGTTAGCC164172.3074955303430675No Hit
TGCGTGAA158672.230190143141466No Hit
CTAGGAAC155202.1814174715797283No Hit
AGCATTGG142822.007410072751397No Hit
TTACCGAC139881.9660868294109048No Hit
TTGTGAGC139021.9539990779575638No Hit
CGGTTAAC137161.9278558015584768No Hit
ATACGTCC133011.869525373033632No Hit
AAGGTACC132761.8660114917971957No Hit
CATAGACC131681.8508315248557905No Hit
GCAGAGAA130201.8300293479360865No Hit
CTAGAGCT126981.7847705576107857No Hit
CGTCGCTA124181.7454150877626977No Hit
TCGTGGAA115721.6265053467216894No Hit
TAGTGTAG114841.6141364847694333No Hit
ATGGATGC113761.5989565178280276No Hit
ACGACGTG110471.5527138407565246No Hit
TGCGTACG101461.426073560995356No Hit
ATATACAC96951.362683143490043No Hit
AGACGGAA84321.1851618634252752TruSeq Adapter, Index 14 (100% over 8bp)
CTGACTTC80121.1261286586531434No Hit
GCTCTAGT79021.110667581212823No Hit
GACACTGA68290.959851798544972No Hit
CAATGGTC48940.6878773908447933No Hit
CTGTGCTA47660.6698863189142388No Hit
GTGAACCA46930.6596257857038444No Hit
AGGTTGCA42770.6011548019295425No Hit
AGCTAGAG42570.5983436969403932No Hit
GGCCATAA40930.5752926360293704No Hit
TCGAAGTC32350.4546962319948725No Hit
TCTCTGGA29660.4168868698908167No Hit
GTGCTGAA27840.39130581448955953No Hit
AAGGAGTA27560.3873702675047508ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
AGTCCTTC25410.35715088887139756No Hit
AGAGTAGA21340.29994490234221266No Hit
ATCGTACG19960.28054827791708364RNA PCR Primer, Index 22 (100% over 8bp)
TAGCGAGT19180.269584968459402No Hit
CGTGAGTG18700.2628383164854441No Hit
TCATCGAG18200.25581055401257125No Hit
GGATATCT18070.25398333576962434No Hit
GTAAGGAG17970.25257778327504976No Hit
CTGCGTGT17650.24808001529241114No Hit
ACTATCTG17120.24063058707116594No Hit
TCAGAGAA16680.23444615609503786No Hit
TATCCTCT16630.23374337984775054No Hit
GACACCGT16060.22573173062867552No Hit
ACGCACAA15420.2167361946633983No Hit
GTCACAAC15150.21294120292804697No Hit
CTTGAGGA14510.20394566696276972No Hit
CTAGTATG14040.19733957023826926No Hit
GTCAGATA13840.19452846524912012No Hit
ACGCGTGA13830.19438790999966266No Hit
CTACTATA13070.18370571104089595No Hit
TCTACACT12900.1813162718001192Illumina Single End PCR Primer 1 (100% over 8bp)
ACTGCATA12810.18005127455500208No Hit
TACGAGAC12570.17667794856802313TruSeq Adapter, Index 14 (100% over 8bp)
TCGACGAG12530.17611572757019328No Hit
CGTTACTA12470.17527239607344855No Hit
AGAGTCAC12010.16880685459840553No Hit
GATAGCGT11340.15938965288475593No Hit
TGTCGAAC10760.15123744841622344No Hit
ACGTCTCG10750.151096893166766No Hit
GTTCGAGA10650.14969134067219142No Hit
GTGATAGG10610.1491291196743616No Hit
GTTAGCTC10590.1488480091754467No Hit
CGATCTAC10350.14547468318846773No Hit
TGTAATGA10250.14406913069389315No Hit
AAGCAGCA10220.14364746494552078No Hit
GTCTAGTG10100.1419608019520313No Hit
TGCGTCAC10020.14083635995637164No Hit
CTCTCTAT9720.13661970247264796ABI Solid3 Adapter B (100% over 8bp)
TACTAGGT9510.13366804223404136No Hit
TCTCTAGT9380.13184082399109442No Hit
AACTCTCG9360.13155971349217951TruSeq Adapter, Index 13 (100% over 8bp)
GATCGTGT9120.12818638750520056No Hit
TAGATCGC8340.11722307804751893No Hit
CTAAGCCT8320.116941967548604No Hit
TACACTGA8070.11342808631216758No Hit
ATTGACAT7900.11103864707139083RNA PCR Primer, Index 15 (100% over 8bp)
TCACGGTT7470.10499477134472018No Hit
CACGTCAG7290.10246477685448595No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)