FastQCFastQC Report
Fri 19 Jan 2024
000000000-DNCDK_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DNCDK_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711464
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCA414935.832058965738252No Hit
AAGGACAC397675.589460605174682No Hit
AGCACCTC317424.461504728278592No Hit
TGGCTTCA283473.984319656370526No Hit
AGCCATGC281983.963376924201365No Hit
ACAGATTC263183.6991330552213464No Hit
TCTTCACA210522.958969111578379No Hit
AATCCGTC206512.9026064565459393No Hit
ATCATTCC202262.84287047552652No Hit
ACACGACC190432.6765936154183487No Hit
TCCGTCTA149212.097224877154712No Hit
TATCAGCA130051.8279210191942248No Hit
TAGTCTCC113451.5945993050948466No Hit
TGAGTACG93711.3171432426658272No Hit
ACTACGAC91291.2831288722971226No Hit
CGAGCGAC85211.197671280626989No Hit
GACATAGT72371.0171983403236144No Hit
ACTCACTG71311.0022994838811239No Hit
GTCTGCTA70620.9926011716685594No Hit
ACGCTACT70240.987260072189176No Hit
CGAGAGTT67040.94228239236279TruSeq Adapter, Index 13 (100% over 8bp)
CTGCGTAG53820.7564683525800322No Hit
TCCTGAGC49530.6961701505627832No Hit
GTCTATGA48520.6819740703675801No Hit
GTAGAGGA41610.5848503929924775No Hit
AAGAGGCA40690.5719193100423915No Hit
GCTACGCT39730.5584260060944756No Hit
AGGCAGAA36800.5172433180034408No Hit
CATTACGG36360.5110588870273127No Hit
CTCTCTAC35630.5007983538169183No Hit
CGAGGCTG34930.4909594863548964No Hit
CAGAGAGG34650.4870239393700876No Hit
GGACTCCT33210.4667839834482138No Hit
TAAGGCGA31890.4482306905198295No Hit
TAGGCATG31300.43993793080183957No Hit
GATTCGGA30470.42827184509687066No Hit
GAACCACA30390.427147403101211No Hit
ACCGGTAA28510.40072301620320916No Hit
ACAGTGGT28170.3959441377216556No Hit
GCGACTAA26090.36670864583450463No Hit
CGTGTACA24690.3470309109104607No Hit
GCATAAGG24390.342814253426737No Hit
CATCTTCG23300.3274937312358742No Hit
AACCTCCA21970.3087998830580324No Hit
GGTTGCAA21430.3012098995873298No Hit
CACTAGCT21300.29938268134438284Illumina PCR Primer Index 10 (100% over 8bp)
CGTACTAG18280.2569349960082309No Hit
ATAGTACC17360.2440039130581449No Hit
ACGTGCGC16830.23655448483689967No Hit
TAGCAGCT15970.22446673338355844No Hit
TATAGCGA15690.22053118639874963No Hit
CTCGACTT14890.20928676644215308No Hit
CGAAGTAT14420.2026806697176526No Hit
GCTCATGA14370.20197789347036532No Hit
GCGTAGTA13140.18468959778709815No Hit
ACTCGCTA13130.1845490425376407No Hit
TCTCTATG12670.17808350106259768ABI Solid3 Adapter B (100% over 8bp)
TGCTCGTA12500.17569406182182093No Hit
AACGCTGA10830.15222133516242564No Hit
CTACGACC10770.1513780036656809No Hit
CGGAGCCT10730.1508157826678511No Hit
ATCTCAGG9650.13563581572644576No Hit
CCTAAGAC9590.134792484229701No Hit
ATGAGCTC9480.13324637648566898No Hit
TTCCCTCC9440.13268415548783916No Hit
TACTAGGT9390.13198137924055187No Hit
CGTAGCGA9200.1293108295008602No Hit
TAACGTCC9100.12790527700628562No Hit
ACTGAGCG9070.12748361125791324No Hit
AGCATACC9010.12664027976116854No Hit
GATCTACG8890.12495361676767903No Hit
GCGTATAC8850.1243913957698492No Hit
CGTCATAC8560.12031529353558297No Hit
TTTTTTTT8280.11637974655077418No Hit
GGAGCTAC8280.11637974655077418No Hit
ACGTACGT8200.11525530455511453No Hit
AGTCGCAG8170.11483363880674215No Hit
GTTACAGC8170.11483363880674215No Hit
TACGCTGC8040.11300642056379523No Hit
CGCGATAT7930.11146031281976321No Hit
TAGCGCTC7900.11103864707139083No Hit
TCCTCATG7870.11061698132301845No Hit
GAGACTTA7310.10274588735340087No Hit
GTAACGAG7290.10246477685448595No Hit
CGATCAGT7240.10176200060719867No Hit
ATAGCGCT7220.10148089010828376No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)