FastQCFastQC Report
Fri 2 Feb 2024
000000000-DN4P6_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DN4P6_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671700
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC583948.693464344201281Illumina Single End PCR Primer 1 (100% over 8bp)
AGCATTGG198392.9535506922733363No Hit
AGACGGAA187292.7882983474765517TruSeq Adapter, Index 14 (100% over 8bp)
CGGTTAAC176252.6239392585975883No Hit
AGTTCAGG167952.5003721899657583No Hit
TTGCCTGA165962.47074586869138No Hit
CCGTAACA158962.3665326782789937No Hit
CATAGACC155152.3098109274973946No Hit
CTAGGAAC154942.3066845317850233No Hit
TGTAGGTG152892.276164954592824No Hit
TTACCGAC150362.2384993300580613No Hit
TGTTAGCC147712.199047193687658No Hit
CTGACTTC145782.1703141283311003No Hit
TGCGTGAA142622.123269316659223No Hit
ATACGTCC140632.0936429953848443No Hit
ATAGCCTC140012.084412684234033No Hit
TCCAACAG139872.0823284204257853No Hit
CACTGAGA127581.8993598332588952No Hit
AAGGTACC126891.8890873902039602No Hit
TCGTGGAA124571.854548161381569No Hit
GCAGAGAA113761.6936132201875838No Hit
ATGGATGC110641.64716391246092No Hit
TTGTGAGC109511.630340926008635No Hit
GTGATAGG92041.370254577936579No Hit
ACGCACAA85191.2682745273187435No Hit
TGTCGAAC82951.2349263063867797No Hit
CTTGAGGA77321.1511091260979605No Hit
AACTCTCG75591.1253535804674706TruSeq Adapter, Index 13 (100% over 8bp)
GTTCGAGA71111.0586571386035433No Hit
TCAGAGAA70931.055977370850082No Hit
GTCACAAC70571.050617835343159No Hit
CAATGGTC59780.8899806461217805No Hit
TCGAAGTC58940.8774750632722941No Hit
GTTAGCTC58640.8730087836831919No Hit
CTAAGCCT50300.7488462111061486No Hit
CTCTCTAT46970.6992705076671133ABI Solid3 Adapter B (100% over 8bp)
AGAGTAGA42260.6291499181182075No Hit
CGTTACTA40490.6027988685425041No Hit
TATCCTCT39330.5855292541313086No Hit
TACGAGAC38880.5788298347476551TruSeq Adapter, Index 14 (100% over 8bp)
ACTGCATA36510.5435462259937472No Hit
AAGGAGTA33630.5006699419383653ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
GTCAGATA32900.48980199493821647No Hit
AGAGTCAC31010.46166443352687214No Hit
TCGACGAG30710.4571981539377698No Hit
GATCGTGT29410.4378442757183266No Hit
GTAAGGAG27130.40390055084114934No Hit
CTACTATA26410.39318147982730384No Hit
ACGTCTCG26370.3925859758820902No Hit
TAGATCGC26250.3907994640464493No Hit
TTGATAGG15030.22376060741402412No Hit
TTCACAAC12420.18490397498883432No Hit
TTTCGAGA12230.18207533124906952No Hit
TTTAGCTC10690.1591484293583445No Hit
CCAGAGAA8040.11969629298794104No Hit
AGCCAACT7800.11612326931665921No Hit
ATTGACAT7610.11329462557689446RNA PCR Primer, Index 15 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)