FastQCFastQC Report
Fri 2 Feb 2024
000000000-DN4P6_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DN4P6_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671700
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCC137002.039601012356707No Hit
GAACCACA130391.9411939854101532No Hit
TACGCTGC124651.8557391692719964No Hit
TAGCGCTC124621.855292541313086No Hit
AGCCATGC121611.81048086943576No Hit
ACAGATTC120791.7982730385588803No Hit
AGCACCTC119941.7856185797230906No Hit
ATGCGCAG115471.7190710138454666No Hit
TTCACGCA113661.6921244603245498No Hit
TCCGTCTA109431.6291499181182074No Hit
ACTGAGCG109041.6233437546523746No Hit
TCTTCACA107991.6077117760905166No Hit
GATTCGGA106911.5916331695697485No Hit
TATCAGCA104841.5608158404049426No Hit
CGGAGCCT104461.555158552925413No Hit
AATCCGTC100181.491439630787554No Hit
CACTAGCT98711.4695548608009528Illumina PCR Primer Index 10 (100% over 8bp)
AAGGACAC95051.415066249813905No Hit
GCGTAGTA90661.3497096918267084No Hit
AACCTCCA89811.3370552329909187No Hit
ATCTCAGG87921.308917671579574No Hit
TGGCTTCA87571.303707012058955No Hit
GGAGCTAC81851.2185499478934048No Hit
GCTCATGA81661.2157213041536399No Hit
GCGACTAA80781.20262021735894No Hit
ACTCGCTA78611.1703141283311003No Hit
ATAGCGCT76451.1381569152895639No Hit
GCATAAGG73091.0881345838916183No Hit
GGTTGCAA68351.0175673663838023No Hit
ACCGGTAA68131.0142920946851273No Hit
ACACGACC68101.0138454667262171No Hit
CATTACGG67090.9988089921095727No Hit
CAGTAGGT64990.9675450349858568No Hit
ACAGTGGT63850.9505731725472681No Hit
CATCTTCG59790.8901295221080839No Hit
CGTGTACA55340.8238797082030668No Hit
ACACTACC44810.6671132946255769No Hit
ACATCCGA40710.6060741402411791No Hit
ACCGTTAA38200.5687062676790233No Hit
GTAGAGGA38020.566026499925562No Hit
AATGACAC36740.5469703736787257No Hit
TGTCTTCA36530.543843977966354No Hit
CTCTAGAG36270.5399732023224654No Hit
CGAGGCTG35690.5313383951168676No Hit
AGCATACC35130.5230013398838768No Hit
AAGAGGCA34800.5180884323358642No Hit
CGTACTAG33600.5002233139794551No Hit
CCTAAGAC30190.44945660264999254No Hit
GCTACGCT29120.43352687211552776No Hit
CTCTCTAC26850.39973202322465384No Hit
GCTACTAA26600.39601012356706866No Hit
CAGAGAGG25520.3799315170463004No Hit
ATGAGCTC24710.3678725621557243No Hit
TAAGGCGA24110.35894000297751977No Hit
GGACTCCT23600.3513473276760458No Hit
TCCTGAGC23000.3424147684978413No Hit
AGGCAGAA22440.33407771326485036No Hit
GGTTTCAA21330.31755247878517195No Hit
CTCAAGTG17590.2618728599076969No Hit
AGAGACCA17150.25532231651034687No Hit
TAGGCATG17080.25428018460622304No Hit
CACTAGCC14200.21140390055084113No Hit
CCAAGCAA13670.20351347327676045No Hit
AACGACAC10330.15378889385142175No Hit
GCCACTAA10110.15051362215274677No Hit
TGCCTTCA10000.14887598630340926No Hit
ACAGTGGC9200.13696590739913653No Hit
GCATAAGT8510.12669346434420128No Hit
AGCATTCC8490.12639571237159447No Hit
CCAATCAA8340.12416257257704333No Hit
ACCATTCC8280.12326931665922287No Hit
TGCACGCA7640.11374125353580467No Hit
TCCTCACA7640.11374125353580467No Hit
TCCACGCA7520.11195474170016377No Hit
CTGAGCCT7500.11165698972755694No Hit
GCATAAGC7410.11031710585082627No Hit
TCGCGCTC7400.11016822986452285No Hit
AAACCGTC7140.1062974542206342No Hit
CTGCGCAG6800.10123567068631831No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)