FastQCFastQC Report
Fri 2 Feb 2024
000000000-DN4P6_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DN4P6_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671700
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG15188322.61173142772071No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT12151718.09096322763138No Hit
GTAACAGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT7396511.011612326931667No Hit
GTAACCGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT56840.8462111061485783No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG49700.739913651927944No Hit
GGCTTGGTCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG33350.49650141432186984No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG32040.47699866011612324No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG30520.4543695101980051No Hit
GTAACAGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT27110.40360279886854256No Hit
CTGTAAAGATAAATGGGTCATCTACAGAGTATGGAGCTGGGATTGACTCG21140.3147238350454072No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20790.30951317552478785No Hit
GTAACAGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC17380.2587464641953253No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACCTCT15090.22465386333184456No Hit
GTAACCGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC12510.186243858865565No Hit
CTGTAAAGATAAATGGGTCATCTAATCCGGTTACCTGTCTCTTATACACA12180.18133095131755247No Hit
CTGTAAAGATAAATGGGTCATCTAATCCTGTTACCTGTCTCTTATACACA11410.16986750037218998No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGCCTCG11330.1686764924817627No Hit
GTAACCGGATTAGAAGGACTGTCTCTTATACACATCTCCGAGCCCACGAG9920.14768497841298198No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGCCTCG9040.13458389161828196No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC8730.1299687360428763No Hit
GTAACCGGTTCAGAAGGACTGTCTCTTATACACATCTCCGAGCCCACGAG8280.12326931665922287No Hit
CTGTAAAGATAAATGGGTCATCTACAGCGTATGGAGCTGGGATTGACTCG7780.1158255173440524No Hit
GTAACAGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACCTCT7530.11210361768646718No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACTCTC7480.11135923775495013No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG7340.1092749739467024No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTATCTCTTATACACATCT6960.10361768646717286No Hit
GGCTTGGTCATTTAGAGGAAGTAGAAGTCGTAACAAGGTTTCCGTAGGTG6870.10227780259044217No Hit
GGCTTGGCATCGATGAAGAACGCAGCCGCTGTCTCTTATACACATCTCCG6790.10108679470001489No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC6760.10064016674110467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)