FastQCFastQC Report
Sat 25 Mar 2023
000000000-DKV2Y_l01_n02_D09_ATH.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DKV2Y_l01_n02_D09_ATH.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGCAGAGACTGGCTATATAAAGGAATGCCATAAAATATTTTCCCTCA12.5No Hit
GGATAGCTATACTTATTCGGTAGACTCTAAAAATACCCTTGGTACTTTAT12.5No Hit
GTAGTGTGACACATGTTTTAGCAAATCGTTACGGAACACAAAACACAGAA12.5No Hit
CTTTTCCTGCTAGGATTGGAAATCCTGTATTTTACATATCCATACGATTG12.5No Hit
CATGTGGAGATTAACACCATTACTCTTGTCTTCTTTTTGTATAACTCTCT12.5No Hit
CTACTTCTTAGTGCATTTGGAACTCATCTAGCATAAAAAATAACAACACT12.5No Hit
CTTCTATACTTTTGGTTCCATTTTGAGTGTAGATAATGACATTGGTTTTG12.5No Hit
GTGATATTCAGTTTGTTGGAAACATCCGTGGCAAACTTTTCGGCCATCTC12.5No Hit
GTCACGCATGGCAACAGGCACAAAATCATGCATAGCTCGAGTAAAGTCTT12.5No Hit
GGGTGGTTTTGATGTTCCAATTGAAGATCTACTTCCTTTTGACTTTGATG12.5No Hit
ATCAAGAACAACAAGTGTTCTCTTCTTGTTTAATTCGTCCCTAGGGAAGC12.5No Hit
AAGCACCTAAGTATGGAACTCCTCGTCTTTTGCTCTCTTTTATAGCTACA12.5No Hit
ATATCATATTTATGGAATACGATTCACTTTCAAGATGCCTTGGTGGTGAA12.5No Hit
GAAGTAGTTTGACAACTGTTTTAGCAAATTGAATGAAACGCACAAACACG12.5No Hit
CCTTTTAGAATCACTTGACTTAGTATAATCTTGGCTAGATCTCATCTGTT12.5No Hit
TGTACACACCGCCCGTCACACTATGGGAGCTGGCCATGCCCGAAGTCGTT12.5No Hit
TTATTATCCTCGGCCCAAACAAAACCATATTTGGCCCAATAACTTAGACC12.5No Hit
GCGGTCCATGATCCTGGTAGTGGAGTTGGTCCTGGGGACAAGGATGCACC12.5No Hit
AGCCTGCTCGGGGCGAGTGGGAGCCCGAACCAGGCCACCAAGCCAACAAC12.5No Hit
CTACGCTTCTTCAAGCTGGTGTTTGGATGAGTTGGTGGAGATTTTCAAAT12.5No Hit
ATGATAGTATCTTGTACCTGAACCGGTGGCTCACTTTTTCTAAGTAATGG12.5No Hit
GGACTGATGAAGCTGCTATGCTTGAAAAACTTTCCACAACTGTCTCAAAC12.5No Hit
TGTGAATAGTTTACTATCTTTTATCTTCTTATATAGTCTATATTTTGTAT12.5No Hit
GGGATGGTGGGAATGGAAGCTCACTTGAGAAAGGCAATCTCGTATTTACG12.5No Hit
CTTCCCTCCTCTGCTAAAAGAACAATGTTCAAGAACAGTCGCCGCAACTC12.5No Hit
GTGAAACAATAGGAAATGTAACTCTACATATATATGTATCTGATTTGTGT12.5No Hit
TAACTGGCTAGACCTTCCTCTCGTGTAACAAACTTTGTCTCCATAGTAGG12.5No Hit
CTCCAGAGGCTTGTCATGTTGAATTTGAAAAACTGCAAAATGTTGTCATG12.5No Hit
CCTACAACCTCTTAGGCTAAGCTTTTCAAGTTTTTGAAGCTTATAAATAG12.5No Hit
ACATTCCCTCGAAAAAGTTGTATTCCGAAGAAGTTGGTCTCTCAATTCAG12.5No Hit
GGGTCTTTCTCCCTTGGGGATAAGGCAAATATTAACCTCGTTTACTGCCG12.5No Hit
GTGTCATGCCTCTGTCTATAGCTCAGAAGATGGGGTACACTGAATTTATG12.5No Hit
CTAATCAAGCTTAGTCATGTACATGTGTCTTCTACTCTTCTTTGTATTAA12.5No Hit
ATTGTCATGAGTCGGGCCATATCAAGCCCAACTGTCCTAAGTTGGCGAGG12.5No Hit
GAACATACCTTGGCTGTGCCACGGTATCGCACAATATTGGGATGCTGAAG12.5No Hit
GTGTCCAACACTCGATCAGGTACTTGACTTTATCACCCACTCGACCATCA12.5No Hit
GTGGTAAGATGGGGAAAGCTGTGATCAATGCGGCGGATTCAGCAGGAGTG12.5No Hit
GGTTGAAGTAGAAGATAGGCTTGAAAATGGATTCTATTTGTAACGCTTGT12.5No Hit
GTTATAACCGATTCGAATCCGATCCGATGATAGTTTGAATCAATATTGGA12.5No Hit
CCTTTGAACAGATGTTCAGCATATCCGTCTCGAATTATCAAGCGGAGCAT12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)