FastQCFastQC Report
Fri 14 Oct 2022
000000000-DJ385_l01.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DJ385_l01.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800120
Sequences flagged as poor quality0
Sequence length251
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA161223889.56280692398283No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA48670.27037086416461126No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA44910.24948336777548163No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA35460.19698686754216382No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA33050.18359887118636536No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA29590.1643779303601982No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA29510.1639335155434082No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA28650.1591560562629158No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA25990.1443792636046486No Hit
TGAGTAACCGCAACATAGGGAAAACACAGTTATCCATACTAGTTAGAGAA24960.13865742283847743No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20650.11471457458391662No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA19870.11038153012021422No Hit
TTAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA19030.1057151745439193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)