FastQCFastQC Report
Fri 14 Oct 2022
000000000-DJ385_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DJ385_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800120
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCGAG48997427.218963180232432No Hit
CTGCGTGT18623610.345754727462614No Hit
ACTATCTG18500210.277203741972757No Hit
GGATATCT1635789.08706086260916No Hit
ATCGTACG1464088.13323556207364RNA PCR Primer, Index 22 (100% over 8bp)
TAGCGAGT1042255.789891784992111No Hit
CGTGAGTG1027045.705397417949914No Hit
TCTTTCCC505592.808646090260649Illumina Single End PCR Primer 1 (100% over 8bp)
TCATCGAT55130.3062573606204031No Hit
ACTATCTT49820.27675932715596735No Hit
TTGCGTGT41170.2287069750905495No Hit
TCTATCTG36800.20443081572339622No Hit
CCATCGAG36560.20309757127302625No Hit
TGATATCT34930.19404261938093015TruSeq Adapter, Index 25 (100% over 8bp)
TCATCGCG33840.18798746750216652No Hit
TCATCTAT31070.17259960447081305No Hit
TCATCTAG29790.1654889674021732No Hit
TCATCGTG27110.15060107103970846No Hit
ATTATCTG25580.14210163766859987No Hit
CTCGTACG24740.13743528209230496No Hit
TATCGAGT23540.1307690598404551No Hit
TCATCTCT23230.12904695242539388No Hit
CTGCGTGG22630.12571384129946894No Hit
TTATCGAG22530.12515832277848143No Hit
GCATATCT22520.12510277092638267No Hit
TCATATCT22320.1239917338844077No Hit
TCATCAGT21850.1213807968357665No Hit
ATCTTACG21640.12021420794169277No Hit
TCATGAGT21570.11982534497700154No Hit
TAGCGAGG21020.11676999311157034No Hit
CATCGAGT20070.1114925671621892No Hit
GGCTATCT19720.10954825233873298No Hit
TAGCGTGT19590.10882607826144923No Hit
ATCGTACT19520.108437215296758No Hit
TATCTGTC19010.10560407083972179No Hit
ACTTTCTG18830.10460413750194432No Hit
CCTATCTG18450.10249316712219185No Hit
TCACCGAG18120.10065995600293315No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)