FastQCFastQC Report
Fri 14 Oct 2022
000000000-DJ385_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DJ385_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800120
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCATAC391432.1744661467013313No Hit
GAGACTTA377452.09680465746728No Hit
TAGCAGCT369342.0517521054151944No Hit
ATAGTACC359411.9965891162811369No Hit
AGGCAGAA353711.96492456058485No Hit
TCAGTCTA344391.9131502344288158No Hit
CAGAGAGG342811.9043730417972133No Hit
TGCGTCAA337701.875986045374753No Hit
AGCATACC333251.8512654711908094No Hit
CGAGCGAC331321.840543963735751No Hit
CTCGACTT327201.8176566006710666No Hit
CATCGTGA325781.809768237673044No Hit
TAAGGCGA315651.7534942114970113No Hit
TCTCTATG314131.7450503299780016ABI Solid3 Adapter B (100% over 8bp)
CGAGCTAG313131.7394951447681266No Hit
GACATAGT307991.7109414927893696No Hit
GCGATACG306041.7001088816301135No Hit
ACGCTACT304291.6903873075128324No Hit
TGAGTACG304071.68916516676666No Hit
CGAAGTAT300061.6668888740750616No Hit
TAGTCTCC299391.6631668999844456No Hit
ACTACGAC295731.6428349221163034No Hit
TAACGTCC287891.5992822700708842No Hit
ACTGTGTA281061.5613403550874385No Hit
TCTAGACT279961.5552296513565762No Hit
CTACGACC278461.5468968735417639No Hit
GATCTACG272211.5121769659800457No Hit
CGAGAGTT262251.4568473212896917TruSeq Adapter, Index 13 (100% over 8bp)
AGTCGCAG261501.4526809323822856No Hit
CGTACTAG253951.4107392840477302No Hit
GAGCTCGA253741.4095726951536565No Hit
GTAACGAG250661.3924627247072416No Hit
CTGCGTAG249001.3832411172588495No Hit
GGACTCCT247521.3750194431482345No Hit
GTTACAGC242801.3487989689576252No Hit
TAGGCATG241881.3436881985645401No Hit
ATGAGCTC240551.3362998022354067No Hit
GCTACGCT239791.3320778614759017No Hit
CTCTCTAC228841.2712485834277716No Hit
CGCGATAT222151.2340843943737083No Hit
CTCTAGAG213741.1873652867586606No Hit
GTCTGCTA211681.1759216052263182No Hit
TCCTGAGC209221.162255849610026No Hit
ACTCACTG207581.1531453458658312No Hit
CTATCGTG202681.1259249383374441No Hit
TGCTCGTA191701.0649290047330178No Hit
TCCTCATG186991.038764082394507No Hit
GCGTATAC175680.9759349376708218No Hit
CGAGGCTG175490.9748794524809458No Hit
ACGTGCGC158720.8817189965113437No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)