MultiQC Report

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

General Statistics

Showing ⁹⁰/₉₀ rows and ³/₅ columns.

Sample Name	% Dups	% GC	Length	% Failed	M Seqs
000000000-D798V_l01_n01_ms_CEP350_G1wt	98.5%	51%	131	50%	0.0
000000000-D798V_l01_n01_ms_CEP350_G2mut	97.9%	50%	131	50%	0.0
000000000-D798V_l01_n01_ms_CEP350_NT	97.2%	50%	131	50%	0.0
000000000-D798V_l01_n01_ms_CEP350_WT	97.6%	50%	131	50%	0.0
000000000-D798V_l01_n01_ms_DENND5A_G1wt	96.1%	52%	131	50%	0.0
000000000-D798V_l01_n01_ms_DENND5A_G2mut	96.1%	52%	131	42%	0.0
000000000-D798V_l01_n01_ms_DENND5A_NT	95.6%	52%	131	50%	0.0
000000000-D798V_l01_n01_ms_DENND5A_WT	96.1%	52%	131	50%	0.0
000000000-D798V_l01_n01_ms_DISL3_G1wt	97.1%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_DISL3_G2mut	97.0%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_DISL3_NT	97.8%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_DISL3_WT	97.4%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_FNDC3A_G1wt	97.6%	41%	131	50%	0.0
000000000-D798V_l01_n01_ms_FNDC3A_G2mut	98.6%	40%	131	42%	0.0
000000000-D798V_l01_n01_ms_FNDC3A_NT	97.9%	40%	131	42%	0.0
000000000-D798V_l01_n01_ms_FNDC3A_WT	98.8%	40%	131	42%	0.0
000000000-D798V_l01_n01_ms_HEATR1_G1wt	97.6%	47%	131	50%	0.0
000000000-D798V_l01_n01_ms_HEATR1_G2mut	97.5%	46%	131	42%	0.0
000000000-D798V_l01_n01_ms_HEATR1_NT	97.0%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_HEATR1_WT	97.7%	46%	131	50%	0.0
000000000-D798V_l01_n01_ms_IMMT_G1wt	98.5%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_IMMT_G2mut	96.4%	44%	131	50%	0.0
000000000-D798V_l01_n01_ms_IMMT_NT	98.5%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_IMMT_WT	99.1%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_NSFL1C_G1wt	96.9%	48%	131	50%	0.0
000000000-D798V_l01_n01_ms_NSFL1C_G2mut	97.4%	48%	131	50%	0.0
000000000-D798V_l01_n01_ms_NSFL1C_NT	97.4%	48%	131	50%	0.0
000000000-D798V_l01_n01_ms_NSFL1C_WT	95.5%	47%	131	50%	0.0
000000000-D798V_l01_n01_ms_PIK3C2_G1wt	98.5%	41%	131	50%	0.0
000000000-D798V_l01_n01_ms_PIK3C2_G2mut	98.7%	41%	131	50%	0.0
000000000-D798V_l01_n01_ms_PIK3C2_NT	98.4%	41%	131	50%	0.0
000000000-D798V_l01_n01_ms_PIK3C2_WT	98.6%	41%	131	50%	0.0
000000000-D798V_l01_n01_ms_QSER1_G1wt	97.8%	43%	131	42%	0.0
000000000-D798V_l01_n01_ms_QSER1_G2mut	92.5%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_QSER1_NT	97.8%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_QSER1_WT	97.9%	43%	131	50%	0.0
000000000-D798V_l01_n01_ms_RAB3GAP1_G1wt	98.4%	44%	131	42%	0.0
000000000-D798V_l01_n01_ms_RAB3GAP1_G2mut	97.8%	44%	131	50%	0.0
000000000-D798V_l01_n01_ms_RAB3GAP1_NT	97.8%	44%	131	50%	0.0
000000000-D798V_l01_n01_ms_RAB3GAP1_WT	98.2%	44%	131	50%	0.0
000000000-D798V_l01_n01_ms_RNPEP_G1wt	98.5%	51%	131	50%	0.0
000000000-D798V_l01_n01_ms_RNPEP_G2mut	98.2%	51%	131	50%	0.0
000000000-D798V_l01_n01_ms_RNPEP_NT	98.6%	51%	131	50%	0.0
000000000-D798V_l01_n01_ms_RNPEP_WT	98.5%	51%	131	50%	0.0
000000000-D798V_l01_n01_undetermined	78.0%	49%	131	50%	0.1
000000000-D798V_l01_n02_ms_CEP350_G1wt	98.4%	49%	131	50%	0.0
000000000-D798V_l01_n02_ms_CEP350_G2mut	97.4%	50%	131	42%	0.0
000000000-D798V_l01_n02_ms_CEP350_NT	97.0%	50%	131	50%	0.0
000000000-D798V_l01_n02_ms_CEP350_WT	97.0%	51%	131	50%	0.0
000000000-D798V_l01_n02_ms_DENND5A_G1wt	94.8%	49%	131	50%	0.0
000000000-D798V_l01_n02_ms_DENND5A_G2mut	94.8%	50%	131	42%	0.0
000000000-D798V_l01_n02_ms_DENND5A_NT	93.9%	51%	131	50%	0.0
000000000-D798V_l01_n02_ms_DENND5A_WT	94.7%	51%	131	58%	0.0
000000000-D798V_l01_n02_ms_DISL3_G1wt	97.0%	45%	131	50%	0.0
000000000-D798V_l01_n02_ms_DISL3_G2mut	96.8%	46%	131	50%	0.0
000000000-D798V_l01_n02_ms_DISL3_NT	97.6%	46%	131	50%	0.0
000000000-D798V_l01_n02_ms_DISL3_WT	97.2%	46%	131	50%	0.0
000000000-D798V_l01_n02_ms_FNDC3A_G1wt	97.7%	40%	131	50%	0.0
000000000-D798V_l01_n02_ms_FNDC3A_G2mut	98.5%	40%	131	33%	0.0
000000000-D798V_l01_n02_ms_FNDC3A_NT	97.7%	40%	131	42%	0.0
000000000-D798V_l01_n02_ms_FNDC3A_WT	98.5%	40%	131	33%	0.0
000000000-D798V_l01_n02_ms_HEATR1_G1wt	97.7%	47%	131	50%	0.0
000000000-D798V_l01_n02_ms_HEATR1_G2mut	97.3%	46%	131	33%	0.0
000000000-D798V_l01_n02_ms_HEATR1_NT	97.4%	47%	131	50%	0.0
000000000-D798V_l01_n02_ms_HEATR1_WT	97.9%	47%	131	50%	0.0
000000000-D798V_l01_n02_ms_IMMT_G1wt	98.5%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_IMMT_G2mut	96.3%	44%	131	42%	0.0
000000000-D798V_l01_n02_ms_IMMT_NT	98.3%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_IMMT_WT	99.0%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_NSFL1C_G1wt	93.8%	47%	131	50%	0.0
000000000-D798V_l01_n02_ms_NSFL1C_G2mut	94.0%	48%	131	50%	0.0
000000000-D798V_l01_n02_ms_NSFL1C_NT	94.5%	47%	131	50%	0.0
000000000-D798V_l01_n02_ms_NSFL1C_WT	92.6%	48%	131	50%	0.0
000000000-D798V_l01_n02_ms_PIK3C2_G1wt	97.8%	42%	131	50%	0.0
000000000-D798V_l01_n02_ms_PIK3C2_G2mut	97.8%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_PIK3C2_NT	97.8%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_PIK3C2_WT	97.9%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_QSER1_G1wt	93.5%	41%	131	50%	0.0
000000000-D798V_l01_n02_ms_QSER1_G2mut	88.0%	42%	131	42%	0.0
000000000-D798V_l01_n02_ms_QSER1_NT	93.7%	42%	131	42%	0.0
000000000-D798V_l01_n02_ms_QSER1_WT	93.5%	43%	131	50%	0.0
000000000-D798V_l01_n02_ms_RAB3GAP1_G1wt	98.2%	44%	131	42%	0.0
000000000-D798V_l01_n02_ms_RAB3GAP1_G2mut	97.6%	44%	131	50%	0.0
000000000-D798V_l01_n02_ms_RAB3GAP1_NT	97.9%	44%	131	50%	0.0
000000000-D798V_l01_n02_ms_RAB3GAP1_WT	98.0%	44%	131	42%	0.0
000000000-D798V_l01_n02_ms_RNPEP_G1wt	97.6%	51%	131	50%	0.0
000000000-D798V_l01_n02_ms_RNPEP_G2mut	97.4%	51%	131	50%	0.0
000000000-D798V_l01_n02_ms_RNPEP_NT	97.7%	51%	131	50%	0.0
000000000-D798V_l01_n02_ms_RNPEP_WT	97.3%	51%	131	42%	0.0
000000000-D798V_l01_n02_undetermined	69.9%	46%	131	50%	0.1

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Sort	Group	Column	Description	ID	Scale
\|\|	FastQC	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC	Length	Average Sequence Length (bp)	`avg_sequence_length`	None
\|\|	FastQC	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Lane 1 Demultiplexing Report

Lane 1 Demultiplexing Report
Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool

Showing ⁴⁵/₄₅ rows and ²/₂ columns.

Sample Name	Total Read Count	Portion (%)
undetermined_library	68,724	8.0
ms_DISL3_G1wt	31,540	3.6
ms_RAB3GAP1_G1wt	16,055	1.9
ms_HEATR1_G1wt	10,547	1.2
ms_FNDC3A_G1wt	43,848	5.1
ms_PIK3C2_G1wt	26,359	3.0
ms_IMMT_G1wt	19,123	2.2
ms_NSFL1C_G1wt	19,557	2.3
ms_RNPEP_G1wt	16,981	2.0
ms_CEP350_G1wt	17,530	2.0
ms_QSER1_G1wt	15,467	1.8
ms_DENND5A_G1wt	14,252	1.6
ms_DISL3_G2mut	25,400	2.9
ms_RAB3GAP1_G2mut	16,001	1.9
ms_HEATR1_G2mut	11,881	1.4
ms_FNDC3A_G2mut	22,401	2.6
ms_PIK3C2_G2mut	24,197	2.8
ms_IMMT_G2mut	26,541	3.1
ms_NSFL1C_G2mut	22,920	2.7
ms_RNPEP_G2mut	12,062	1.4
ms_CEP350_G2mut	17,469	2.0
ms_QSER1_G2mut	42,783	4.9
ms_DENND5A_G2mut	18,795	2.2
ms_DISL3_NT	19,589	2.3
ms_RAB3GAP1_NT	11,344	1.3
ms_HEATR1_NT	7,457	0.9
ms_FNDC3A_NT	10,459	1.2
ms_PIK3C2_NT	23,753	2.7
ms_IMMT_NT	10,100	1.2
ms_NSFL1C_NT	7,066	0.8
ms_RNPEP_NT	19,159	2.2
ms_CEP350_NT	10,251	1.2
ms_QSER1_NT	16,389	1.9
ms_DENND5A_NT	11,097	1.3
ms_DISL3_WT	21,566	2.5
ms_RAB3GAP1_WT	8,952	1.0
ms_HEATR1_WT	10,543	1.2
ms_FNDC3A_WT	16,983	2.0
ms_PIK3C2_WT	23,608	2.7
ms_IMMT_WT	30,675	3.5
ms_NSFL1C_WT	7,598	0.9
ms_RNPEP_WT	12,980	1.5
ms_CEP350_WT	11,973	1.4
ms_QSER1_WT	17,514	2.0
ms_DENND5A_WT	14,966	1.7

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Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None

Barcodes of Undetermined Reads

Barcodes of Undetermined Reads
We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

Showing ²⁰/₂₀ rows and ²/₂ columns.

Sample Name	Count	Frequency (%)
CTGAGCTAATAGAGGC	533.0	0.8
CTGAGCTATATAGCCT	477.0	0.7
CTGAACTATATAGCCT	392.0	0.6
CTGAACTAATAGAGGC	383.0	0.6
CGGCTAGATATAGCCT	344.0	0.5
CTTCCTCTTCTTTCCC	341.0	0.5
CGGCTAGAATAGAGGC	330.0	0.5
ATTACTCGTCTTTCCC	320.0	0.5
TCCGCGAATCTTTCCC	311.0	0.5
TATGCGCACCTATCCT	273.0	0.4
GAGATTCCTCTTTCCC	269.0	0.4
TCCTTCTCTCTTTCCC	246.0	0.4
CGGCTAGAGGCTCTGA	244.0	0.3
TATGCGCATATAGCCT	243.0	0.3
TAAGCGCACCTATCCT	235.0	0.3
GAATTCGTTCTTTCCC	230.0	0.3
GGGGGGGGGGGGGGGG	230.0	0.3
TGAAGCTAATAGAGGC	224.0	0.3
CGGCTAGACCTATCCT	222.0	0.3
GGCTATGATATAGCCT	216.0	0.3

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Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Count	Count	`Count`	None
\|\|			Frequency (%)	Frequency (%)	`Frequency (%)`	None

Sample Name	Total # of Single-End Reads	Total # PF Reads	% Undetermined	% PhiX Aligned
1.0	917,373	864,455	8.0	3.2

Sort	Column	Description	ID	Scale
\|\|	Total # of Single-End Reads	Total # of Single-End Reads	`Total # of Single-End Reads`	None
\|\|	Total # PF Reads	Total # PF Reads	`Total # PF Reads`	None
\|\|	% Undetermined	% Undetermined	`% Undetermined`	None
\|\|	% PhiX Aligned	% PhiX Aligned	`% PhiX Aligned`	None

FastQC

FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

Sequence Quality Histograms

The mean quality value across each base position in the read. See the FastQC help.

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

Click a heatmap row to see a line plot for that dataset.

rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

Per Base N Content

The percentage of base calls at each position for which an N was called. See the FastQC help.

Sequence Length Distribution

All samples have sequences of a single length (131bp).

Sequence Duplication Levels

The relative level of duplication found for every sequence. See the FastQC help.

Overrepresented sequences

The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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General Statistics

Lane 1 Demultiplexing Report

Barcodes of Undetermined Reads

Lane Statistics

FastQC

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

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Per Sequence GC Content

Per Base N Content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

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General Statistics

General Statistics: Columns

Lane 1 Demultiplexing Report

Table Lydu: Columns

Barcodes of Undetermined Reads

Table Wzvb: Columns

Lane Statistics

Table Ulhw: Columns

FastQC

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

rollover for sample name

Per Sequence GC Content

Per Base N Content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

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