FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68724
Sequences flagged as poor quality0
Sequence length131
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT37775.495896629998254No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT27343.9782317676503114No Hit
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA25823.7570572143647047No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA25823.7570572143647047No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG16992.472207671264769No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC13972.032768756184157No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA13201.9207263837960538No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT12381.8014085326814504No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC12071.7563005645771492No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA11821.7199231709446483No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT11571.6835457773121472No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC10821.574413596414644No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC10711.5584075432163436No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG9791.42453873464874No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT9041.3154065537512367No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA7921.1524358302776323No Hit
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG6250.9094348408125255No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT4580.6664338513474186No Hit
CAATTGCCCACACCAACGCTTAAGTTTCCATCCAAGATCGGAAGAGCGTC3160.45981025551481286No Hit
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTCCAGCACGCTCCGACATG3110.45253477678831266No Hit
CTGAGGTGAAGTGATGAGGCAGATAGATCGGAAGAGCGTCGTGTAGGGAA3030.4408940108259123Illumina Single End PCR Primer 1 (100% over 26bp)
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG2580.37541470228741053No Hit
GTCTGCAAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA2450.35649845759850995Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA2070.30120481927710846No Hit
CTTTTTCTTCCTCTTTCTTCTCTCCCTTTTTTTTTTTTCTTTTTTCTTCC1840.2677376171352075No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA1720.25027646819160704No Hit
CTCTCCTTCCTTTCTTCTCCCCTCTTTTCTCCCTTCTTCTTTTTTCTTTT1270.18479715965310517No Hit
TTCCCTTCCCCCTCTTCCTTCCCCTCTTTCCTCCCTCCTTTCTCTTCTTT1060.15424014900180433No Hit
ACAGGCTTGCATATCAGAGCTATAAGAGTCAGTCTGCCTTTTACATAACT1040.15132995751120426No Hit
CCCTTTCCCCCCCCCCCTCCTCCCTCTTTTCTTTTCTTCTCTTCTCCCCC1020.14841976602060417No Hit
CCCTTCTTTCCTCTCCTCTCTCTCCTCTTCCTTCTTCCTTTTCCCTCCCT900.13095861707700368No Hit
TCTCTTCTCCCCCCCTCCTTTTTTCCCTCTTCTCCCCCCTCTTCCTCCTC860.12513823409580352No Hit
TCTTCCTCTCCCTTTTCTCTTCCTTCTTTCCTTTTCCCCTTTTTTTTTTC810.11786275536930331No Hit
CCCTTTCCCCCCCCCCCTCCTCCCTCTTTTCTTTTCTTCTCTTCTCCTCC770.11204237238810315No Hit
CCTCCTTCCCTTTCCCTCCCTCTTCCTTTTTTTCTTTCTTCCTTCCTCTT730.10622198940690299No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA710.10331179791630289No Hit
TTCCCTTCCCCCTCTTCCTTCCCCTCTTTCCTCCCTCCTTTCTCTTCTTC700.10185670217100284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGAT353.6379788E-11125.000019
CCTTGAT3450.0125.09
CTGCCAG301.8026185E-9125.03
AGACTTC3500.0125.03
GCATATC4250.0125.09
AATTGCC3450.0125.02
TCCTTAG1450.0124.999997
GCCCACA3550.0124.999996
CTTAGGT1450.0124.999999
TGCCCAC3550.0124.999995
TTGCCCA3550.0124.999994
AGCCGAA5800.0123.922418
GCCGAAT5750.0123.913059
AAGCCGA5500.0123.863637
GACCTTC3750.0123.333332
TCCCCAG3750.0123.333337
GACTTCC3600.0123.263894
GAGACTT3550.0123.239432
CAATTGC3450.0123.188411
TCAAGGG2800.0122.767869
Produced by FastQC (version 0.11.5)