FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RNPEP_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RNPEP_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12980
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT859466.2095531587057No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT362027.889060092449924No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT430.3312788906009245No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT370.2850539291217257No Hit
ACTCCTTACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT290.22342064714946072No Hit
ATCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT250.1926040061633282No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT200.15408320493066258No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT160.12326656394453005No Hit
ACTCCTTCACTTTCCAAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT160.12326656394453005No Hit
ACTCCTTCATTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT150.11556240369799693No Hit
ACTCCTTCACTTTCCAGGCATTTGAGATACTTGGAGATCGGAAGAGCGTC150.11556240369799693No Hit
ACTCCTTCACTTTCCAAAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT140.10785824345146379No Hit
CTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT130.10015408320493066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTCA152.171403E-4125.02
TCTTCAC152.171403E-4125.03
TCCTTCA12350.0124.999993
ACTCCTT12450.0124.497991
CCTTCAC12400.0124.495974
CTCCTTC12450.0123.995992
TTCACTT12550.0123.007966
CTTCACT12550.0123.007965
CACTTTC12550.0123.007968
TCACTTT12550.0123.007967
ACTTTCC12600.0122.519849
ACTCTTC206.8196334E-493.750011
CTTCCTC150.003448230262.562-63
GGATGAT3950.062.49999642-43
ATGATTT3950.062.49999644-45
AAGAGTG500.062.499996124-125
AAGGATG3950.062.49999640-41
GCCCCAT12600.062.25198452-53
CCGGAGC12600.062.25198464-65
GTTCTTA12600.062.25198434-35
Produced by FastQC (version 0.11.5)