FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RNPEP_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RNPEP_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19159
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT1277666.68406493031995No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT529227.621483375959077No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT550.28707135027924213No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT480.2505349966073386No Hit
ATCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT410.21399864293543502No Hit
ACTCCTTCACTTTCCAGGCATTTGAGATACTTGGAGATCGGAAGAGCGTC390.20355968474346262No Hit
ACTCCTTACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT350.18268176835951772No Hit
ACTCCTTCACTTTCCAAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT220.11482854011169687No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT200.1043895819197244No Hit
ACTCCTTCATTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT200.1043895819197244No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT200.1043895819197244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATT204.346888E-6125.000015
TTCATTT204.346888E-6125.000016
CCTTCAT204.346888E-6125.000014
CCTTCAC18450.0125.000014
TCCTTCA18650.0125.03
CTCCTTC18650.0125.02
CTCTTCA258.6636646E-8125.02
ACTCTTC258.6636646E-8125.01
CATTTCC152.1876661E-4125.08
ACTTTCA152.1876661E-4125.09
TCATTTC152.1876661E-4125.07
TCTTCAC258.6636646E-8125.03
ACTCCTT18700.0124.999991
TTCACTT18700.0123.328876
CTTCACT18700.0123.328875
CACTTTC18700.0123.328878
TCACTTT18700.0123.328877
ACTTTCC18600.0123.319899
ATTTCCA206.870492E-493.750019
GCCCCAT18800.062.50000452-53
Produced by FastQC (version 0.11.5)