FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RNPEP_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RNPEP_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12062
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT858371.15735367269109No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT279423.16365445199801No Hit
ACTCCTTCACTTTCCAGGCATTTGAGATACTTGGAGATCGGAAGAGCGTC410.33991046260984914No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT360.29845796716962364No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT340.2818769689935334No Hit
ATCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT180.14922898358481182No Hit
ACTCCTTCACTTTCCAAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT160.1326479854087216No Hit
ACTCCTTCACTTTCCGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT140.11606698723263141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTT11750.0125.06
CTTCACT11750.0125.05
TCCTTCA11750.0125.03
CTCCTTC11750.0125.02
ACTCCTT11800.0125.01
CACTTTC11800.0125.08
CCTTCAC11750.0125.04
TCACTTT11850.0124.472577
ACTTTCC11800.0124.470349
GGATGAT3250.062.50000442-43
ATGATTT3250.062.50000444-45
TGATTTG3250.062.50000446-47
GATGATT3250.062.50000444-45
GCCCCAT11750.062.552-53
CTCTGCA11750.062.570-71
AATCTTC11700.062.518-19
GGCATTT11700.062.582-83
CCGGAGC11750.062.564-65
AGCTCTG11800.062.568-69
GAAATCT11600.062.516-17
Produced by FastQC (version 0.11.5)