FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RNPEP_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RNPEP_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16981
Sequences flagged as poor quality0
Sequence length131
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT1019960.06124492079383No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT575933.914374889582476No Hit
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT460.2708909958188564No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT400.23555738766857076No Hit
ACTCCTTCACTTTCCAGGCATTTGAGATACTTGGAGATCGGAAGAGCGTC330.19433484482657087No Hit
ACTCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT320.1884459101348566No Hit
ACTCCTTACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT230.1354454979094282No Hit
ATCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATTT220.12955656321771392No Hit
GTCTGCAAGCCGAATTTGAGATACTTGGAGATCGGAAGAGCGTCGTGTAG220.12955656321771392Illumina Single End PCR Primer 1 (100% over 22bp)
ACTCCTTCACTTTCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATTT170.10011188975914258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCTT16600.0125.000011
ACTCTTC152.1832493E-4125.000011
TCCTTCA16550.0125.03
CTCCTTC16550.0125.02
CCTTCAC16550.0125.04
TTCACTT16750.0123.880616
CTTCACT16750.0123.880615
TCACTTT16800.0123.511917
ACTTTCC16750.0123.507479
CACTTTC16750.0123.507478
CTCTTCA206.856683E-493.749992
TCTTCAC206.856683E-493.749993
TTTCCAG16650.062.50000410-11
ACGATTT10000.062.544-45
AAGGACG10000.062.540-41
GGACGAT10050.062.542-43
TCCAGAA16600.062.3117512-13
CAGAAAT16500.062.31060814-15
GAAATCT16450.062.1200616-17
GCAGCCG16750.061.94030460-61
Produced by FastQC (version 0.11.5)