FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RAB3GAP1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RAB3GAP1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8952
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT558962.43297587131368No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT300533.56791778373548No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA260.290437890974084No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA170.18990169794459338No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTTA150.1675603217158177No Hit
GCCATCTCCCTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT120.13404825737265416No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTTA120.13404825737265416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAG8950.0125.07
ATCTCCT8950.0125.04
CTCCTTA8950.0125.06
CATCTCC8950.0125.03
CTTAGGT8950.0125.09
CCATCTC8950.0125.02
GCCATCT8950.0125.01
TCTCCTT8950.0125.05
CCTTAGG9200.0121.6032648
TCCTTTC8950.062.518-19
TCCTTTA8950.062.544-45
CTTCAGT8950.062.556-57
TTGTCCA8950.062.536-37
TCCTCCT8950.062.514-15
GGCATTC8950.062.570-71
CTTTAAG8950.062.546-47
CTCCTTT8950.062.516-17
AAATTCT8950.062.580-81
TTTCTTG8950.062.520-21
TAAGTTC8950.062.550-51
Produced by FastQC (version 0.11.5)