FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RAB3GAP1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RAB3GAP1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT713662.90550070521862No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT370032.616361071932296No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA350.3085331452750353No Hit
GCCATCTCCTTAGGTCCTTCTTCGCTGTCACTCAGATCGGAAGAGCGTCG300.2644569816643159No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTTA190.16748942172073342No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA120.10578279266572638No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTTA120.10578279266572638No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAG11200.0125.000017
CTTAGGT11200.0125.000019
ATCTCCT11150.0125.04
CTCCTTA11150.0125.06
CATCTCC11150.0125.03
CCATCTC11150.0125.02
GCCATCT11150.0125.01
TCTCCTT11200.0124.441975
CCTTAGG11900.0117.6470648
TCCTCCT11100.062.50000414-15
GTCACTA201.3602161E-462.50000496-97
GGCATTC11100.062.50000470-71
GGTCCTC11100.062.50000412-13
CTCTCTT11100.062.50000430-31
CTCTTGT11100.062.50000432-33
GTATCCC700.062.50000460-61
CCTCCTT11100.062.50000416-17
GTCCTCC11100.062.50000414-15
TATCCCT700.062.50000462-63
TCTTGTC11100.062.50000434-35
Produced by FastQC (version 0.11.5)