FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RAB3GAP1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RAB3GAP1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16001
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT1074467.1458033872883No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT429426.83582276107743No Hit
GCCATCTCCTTAGGTCCTCCTTTCTCGCCACTCTCTTGTCCATTTCCTTT400.24998437597650147No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTTCCACTCTCTTGTCCATTTCCTTT360.2249859383788513No Hit
GCCATCTCCCTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT310.19373789138178865No Hit
GCCATCTCCTTAGGTCCTTCTTCGCTGTCACTCAGATCGGAAGAGCGTCG290.18123867258296356No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA290.18123867258296356No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTTA280.17498906318355104No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG230.14374101618648835No Hit
GCCATCTCCTTAGGTCCTCCTTTCTCGCCACTCTCTTGTCCACTTCCTTT210.13124179738766328No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA190.11874257858883819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATCT15700.0125.000011
CCATCTC15700.0124.601922
ATCTCCT15750.0124.206354
TCCTTAG15700.0124.203837
CTCCTTA15700.0124.203836
CATCTCC15700.0124.203833
TCTCCTT15750.0123.8095255
CTTAGGT15850.0123.4227149
CCTTAGG16350.0119.266068
TCCTTTA15800.062.50000444-45
TCCTCCT15800.062.50000414-15
GGCATTC15700.062.50000470-71
TTTCTTG15700.062.50000420-21
CAGTATC15800.062.50000458-59
TGTCCAC4500.062.50000436-37
GTTCTTC15700.062.50000452-53
CTCTCTT15800.062.50000430-31
CTTAGGC15700.062.50000466-67
CACTCTC15800.062.50000428-29
CACTTCC4500.062.50000440-41
Produced by FastQC (version 0.11.5)