FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_RAB3GAP1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_RAB3GAP1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16055
Sequences flagged as poor quality0
Sequence length131
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT1001862.39800685144815No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT542233.77141077545936No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTTA370.23045780130800372No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA250.15571473061351604No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA220.1370289629398941No Hit
GCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTTA190.1183431952662722No Hit
GCCATCTCCTTAGGTCCTCCTTTCTCGCCACTCTCTTGTCCATTTCCTTT170.10588601681719091No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCTC15850.0125.02
GCCATCT15850.0125.01
TCCTTAG15850.0124.6056757
ATCTCCT15850.0124.6056754
CTCCTTA15850.0124.6056756
CATCTCC15850.0124.6056753
TCTCCTT15900.0124.213835
CTTAGGT15800.0124.2088559
CCTTAGG16650.0117.867878
TTTCCTT10000.062.50000442-43
CAGTATC15700.062.50000458-59
TGTCCAT9950.062.50000436-37
GTATCCC900.062.50000460-61
CATTTCC10000.062.50000440-41
AGTATCC900.062.50000460-61
TATCCCT900.062.50000462-63
TCCATTT9950.062.50000438-39
TCACCAG201.3689652E-462.50000496-97
TTCAGTA15700.062.50000456-57
CCCTTAG900.062.50000464-65
Produced by FastQC (version 0.11.5)