FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_QSER1_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_QSER1_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17514
Sequences flagged as poor quality0
Sequence length131
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA1027358.65593239693959No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA507828.993947698983668No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA1030.588100947813178No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA660.3768413840356286No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA510.29119561493662216No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA510.29119561493662216No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG490.2797761790567546No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA450.2569373072970195No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT370.2112595637775494No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA370.2112595637775494No Hit
TCTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA330.1884206920178143No Hit
TCTTACGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA290.16558182025807924No Hit
TCTTAAGATCAAGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA290.16558182025807924No Hit
TCTTAAGACAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA280.15987210231814547No Hit
TCTTAAGATCAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA260.14845266643827795No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA250.14274294849834418No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA220.1256137946785429No Hit
TCTTAAGATCAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA190.10848464085874159No Hit
TCTTAAGATCAAGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA180.10277492291880781No Hit
TCTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA180.10277492291880781No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAG152.184457E-4125.000017
TCTTAAG16050.0124.221191
TTAAGAT16150.0123.065013
CTTAAGA16250.0122.69232
TAAGATC16150.0122.678024
AAGATCA16150.0122.678025
ATCAAGG16550.0121.2235648
TCAAGGG16500.0121.212139
GATCAAG16400.0121.189027
AGATCAA16350.0121.1773766
AATAAAA250.001664364775.08
GTAGATT201.3706944E-462.500004124-125
TAGAACT150.00345840662.500004124-125
GTAGAAC150.00345840662.500004124-125
TAAATCT150.00345840662.500004124-125
TATATCT358.760253E-962.500004124-125
GTGTATA302.1813503E-762.500004122-123
AAACACC201.3706944E-462.50000472-73
GTGTAGT150.00345840662.500004122-123
GTGTAAA201.3706944E-462.500004122-123
Produced by FastQC (version 0.11.5)