FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_QSER1_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_QSER1_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16389
Sequences flagged as poor quality0
Sequence length131
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA952458.112148392214294No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA484629.568613094148517No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA900.5491488193300385No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA620.37830251998291536No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA520.31728598450179996No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG470.2867777167612423No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA420.2562694490206846No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT410.25016779547257306No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA400.24406614192446155No Hit
TCTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA350.21355787418390385No Hit
TCTTAAGACAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA280.17084629934712306No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA280.17084629934712306No Hit
TCTTAAGATCAAGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA270.16474464579901155No Hit
TCTTAAGATCAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA220.13423637805845384No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA210.1281347245103423No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA210.1281347245103423No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT210.1281347245103423No Hit
TCTTAAGATCAAGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA200.12203307096223077No Hit
TCTTAAGATCAAAGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA200.12203307096223077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGGT152.1818405E-4125.09
TTTTTAG152.1818405E-4125.01
AGATTAA152.1818405E-4125.06
TCTTAAG14750.0124.999991
CTTAAGA14850.0124.158262
TTAAGAT14800.0124.15543
TAAGATC14750.0123.305084
AAGATCA14750.0123.305085
TCAAGGG14550.0123.28189
GATCAAG14750.0122.881357
ATCAAGG14750.0122.881358
AGATCAA14750.0122.881356
TTTTAGA250.001662387575.02
TTTAGAT250.001662387575.03
TCCTTTT150.003456368362.538-39
TTGTCGG5200.062.540-41
GTATGGA150.003456368362.598-99
TGGTTAA9500.062.544-45
GTGTCAC14600.062.554-55
TTCGCTG150.003456368362.590-91
Produced by FastQC (version 0.11.5)