FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_QSER1_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_QSER1_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42783
Sequences flagged as poor quality0
Sequence length131
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA965422.56503751490078No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG932021.784353598391885No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA589613.781174765677957No Hit
TCTTAAGATCAAGGGTACTGAACCAACAACACTTTCTAGATCGGAAGAGC15103.529439263258771No Hit
TCTTAAGATCAAGGGTAGCTGGTGACTGAACCAACAACACTTTCTAGATC11702.734731084776664No Hit
TCTTAAGATCAAGGGTAGCTGCACTGAACCAACAACACTTTCTAGATCGG9812.2929668326204333No Hit
TCTTAAGATCAAGGGTAGCTGCAGTGACTGAACCAACAACACTTTCTAGA4000.934950798214244No Hit
TCTTAAGATCAAGGGTAGCTGTCTTTCCCAACAACACTTTCTAGATCGGA3930.9185891592454948No Hit
TCTTAAGATCAAGGGTAGCTGCGTGACTGAACCAACAACACTTTCTAGAT3550.8297688334151416No Hit
TCTTAAGATCAAGGGCACTGAACCAACAACACTTTCTAGATCGGAAGAGC2840.6638150667321132No Hit
TCTTAAGATCAAGGGTAGGTGACTGAACCAACAACACTTTCTAGATCGGA2440.5703199869106887No Hit
TCTTAAGATCAAGGGTAGCTGCTCTTTCCCAACAACACTTTCTAGATCGG2420.5656452329196175No Hit
TCTTAAGATCAAGGGTAACTGAACCAACAACACTTTCTAGATCGGAAGAG2360.5516209709464039No Hit
TCTTAAGATCAAGGGTAGCTGTCTTTCCCCAACAACACTTTCTAGATCGG2020.47215015309819325No Hit
TCTTAAGATCAAGGGTAGCTGTGACTGAACCAACAACACTTTCTAGATCG1570.3669681882990908No Hit
TCTTAAGATCAAGGGTAGCGTGACTGAACCAACAACACTTTCTAGATCGG1190.27814786246873763No Hit
TCTTAAGATCAAGGGTAGTCTTTCCCAACAACACTTTCTAGATCGGAAGA1070.25009933852231025No Hit
TCTTAAGATCAAGGGTAGCTGCTCTTTCCCCAACAACACTTTCTAGATCG1060.24776196152677463No Hit
TCTTAAGATCAAGGGTAGCTGCACCAACAACACTTTCTAGATCGGAAGAG1010.23607507654909662No Hit
TCTTAAGATCAAGGGTAGCTGGTTACTGAACCAACAACACTTTCTAGATC900.21036392959820488No Hit
CTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAGC830.19400229062945562No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT800.1869901596428488No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGAAGAGC790.1846527826473132No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA660.15426688170535027No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA650.15192950470981464No Hit
TCTTAAGATCAAGGGTAGCTCTTTCCCAACAACACTTTCTAGATCGGAAG640.14959212771427904No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA640.14959212771427904No Hit
TCTTAAGATCAAGGGTAGCTGCATCTTTCCCAACAACACTTTCTAGATCG600.1402426197321366No Hit
TCTTAAGATCAAGGGTAGCTGTCTTTACCAACAACACTTTCTAGATCGGA580.13556786574106539No Hit
TCTTAAGATCAAGGGTAGCTGTCTTTCCCTACCAACAACACTTTCTAGAT570.13323048874552976No Hit
TCTTAAGATCAAGGGTAGCTGCACACTGAACCAACAACACTTTCTAGATC540.12621835775892293No Hit
TCTTAAGATCAAGGGTAGCTGCAACAACACTTTCTAGATCGGAAGAGCGT530.12388098076338731No Hit
TCTTAATATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG510.11920622677231611No Hit
TCTTAAGATCAAGGGTAGCTGTCTTTCACCAACAACACTTTCTAGATCGG450.10518196479910244No Hit
TCTTAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAGC450.10518196479910244No Hit
TCTTAAGATCAAGGGTAGCTGCTCTTTCACTGAACCAACAACACTTTCTA440.10284458780356683No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA430.10050721080803124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAA152.2065084E-4125.03
CTAAGAT258.851748E-8125.02
TACGATC152.2065084E-4125.04
TATCAAG258.851748E-8125.07
ACGATCA152.2065084E-4125.05
TCTTAGA258.851748E-8125.01
CGATCAA152.2065084E-4125.06
TCTTAAG39800.0124.0577851
CTTAAGA40050.0122.971282
TTAAGAT40000.0122.968763
TAAGATC39950.0122.809764
AAGATCA40000.0122.656265
ATCAAGG40550.0121.917398
GATCAAG40300.0121.898267
AGATCAA40250.0121.894426
TCAAGGG40500.0121.6049359
CTTAGAT302.625202E-7104.1666642
TCTAAGA302.625202E-7104.1666641
CTTAATA251.3367953E-5100.02
TTAATAT251.3367953E-5100.03
Produced by FastQC (version 0.11.5)