FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_QSER1_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_QSER1_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15467
Sequences flagged as poor quality0
Sequence length131
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA880356.91472166548135No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA471030.45192991530355No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA780.50429947630439No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT630.40731880778431495No Hit
TCTTAAGATCAAGGGTAGCTGAACCAACAACACTTTCTAGATCGGAAGAG610.3943880519816383No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA600.3879226740803No Hit
CTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA430.2780112497575483No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA410.26508049395487165No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA370.23921898234951833No Hit
TCTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA320.20689209284282667No Hit
TCTTAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA320.20689209284282667No Hit
TCTTAATATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA300.19396133704015No Hit
TCTTAAGACAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA300.19396133704015No Hit
TCTTAAGATCAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA300.19396133704015No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCACTTCCTTT260.16809982543479668No Hit
TCTTAAGATCAAGGGTACTGAACCAACAACACTTTCTAGATCGGAAGAGC230.14870369173078166No Hit
TTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA220.14223831382944332No Hit
TCTTAAGATCAAGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA200.12930755802676666No Hit
TCTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTAA190.12284218012542833No Hit
TCTTACGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA180.11637680222409No Hit
TCTTAAGATAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTAA170.10991142432275167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTAAG204.3199125E-6125.000011
TCTTAAG14050.0125.000011
TTAAAAG152.1795217E-4125.09
CTTAAGA14200.0123.679572
TTAAGAT14450.0122.837373
TAAGATC14400.0122.8298654
AAGATCA14400.0122.8298655
GATCAAG14500.0122.4137957
AGATCAA14450.0122.404846
ATCAAGG14650.0121.587038
TCAAGGG14700.0120.74839
TGGTTAA10000.062.50000444-45
CGAAGAG201.3681554E-462.50000484-85
AGATCGA201.3681554E-462.50000480-81
ATCGAAG201.3681554E-462.50000482-83
TTTAAGA401.3466121E-462.5000042
TTGTTGG9900.062.540-41
TAAAAGG150.00345456162.510-11
AAGGGGG150.00345456162.592-93
TCGGAAA150.00345456162.584-85
Produced by FastQC (version 0.11.5)