FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_PIK3C2_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_PIK3C2_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23608
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG1511364.01643510674347No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG722130.587089122331413No Hit
AGAGACTTCCTTGATTCGACCAGTGACTGGAGTTCAGACGTGGGCTCATC560.23720772619451033TruSeq Adapter, Index 10 (96% over 26bp)
AGAGATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTGA290.12283971535072856No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGCG250.10589630633683496No Hit
AGAGAATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG240.10166045408336156No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGGG240.10166045408336156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACT23250.0125.000011
GAGACTT23250.0125.000012
AGACTTC23300.0124.999993
ACTTCCT23400.0124.4658055
GACTTCC23400.0124.4658054
TTCCTTG23450.0124.2004247
CTTCCTT23450.0124.2004246
TCCTTGA23400.0124.1987158
CCTTGAT23400.0124.1987159
TAGAAGA201.3756494E-462.50000474-75
CCGTGGA201.3756494E-462.500004124-125
ACCATCT23050.062.50000418-19
CATCTTG23050.062.50000420-21
TTCGACC23150.062.50000414-15
CTGGAGT201.3756494E-462.50000426-27
TCTTGTC23050.062.50000422-23
GATTCGA23250.062.50000412-13
GACTGGA201.3756494E-462.50000424-25
AAAATGA23050.062.50000450-51
AGCCGTT201.3756494E-462.500004122-123
Produced by FastQC (version 0.11.5)