FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_PIK3C2_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_PIK3C2_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23753
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG1516963.86140698017092No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG728030.6487601566118No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGGG390.16418978655327748No Hit
AGAGATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTGA330.1389298193912348No Hit
AGAGAATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG260.10945985770218498No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGCG260.10945985770218498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACT23300.0125.01
AGACTTC23400.0124.732913
GAGACTT23400.0124.732912
ACTTCCT23350.0124.732335
GACTTCC23350.0124.732334
CTTCCTT23400.0124.465816
TCCTTGA23500.0124.2021268
TTCCTTG23500.0124.2021267
CCTTGAT23450.0124.200439
GTATGCT7400.062.536-37
GAAGAGT150.003466040162.5108-109
AGGGAAG150.003466040162.5106-107
ATGCTTT7400.062.538-39
GGGAAGA150.003466040162.5106-107
GCTTTAG7400.062.540-41
TAGGAAA255.4851025E-662.499996104-105
AGGAAAG255.4851025E-662.499996106-107
GTAGGAA255.4851025E-662.499996104-105
TTGATTC23550.062.36730610-11
GATTCGA23500.062.3670212-13
Produced by FastQC (version 0.11.5)