FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_PIK3C2_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_PIK3C2_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24197
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG1524763.011943629375544No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG770831.85518865975121No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGCG430.17770798032813984No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGCG300.12398231185684176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACT23550.0125.01
AGACTTC23700.0124.736283
GAGACTT23650.0124.735732
GACTTCC23850.0124.47594
ACTTCCT23800.0124.4747855
CTTCCTT23750.0124.2105266
TCCTTGA23750.0123.947378
CCTTGAT23750.0123.947379
TTCCTTG23750.0123.947377
CATCTTG23950.062.50000420-21
TTCGACC23950.062.50000414-15
GAGTATG23950.062.50000434-35
TCTTGTC23950.062.50000422-23
AAAGGAT23950.062.50000456-57
GATTCGA23850.062.50000412-13
AAAATGA23850.062.50000450-51
GCTTTAG8150.062.50000440-41
TGAAAGG23950.062.50000454-55
GTATGCT8200.062.536-37
GGTGAAA23750.062.546-47
Produced by FastQC (version 0.11.5)