FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_PIK3C2_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_PIK3C2_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26359
Sequences flagged as poor quality0
Sequence length131
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG1695164.30820592586971No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG797330.247733222049394No Hit
AGAGACTTCCTTGATTCGACCGTGACTGGAGTTCAGACGTGGGCTCATCA410.15554459577373952TruSeq Adapter, Index 11 (96% over 27bp)
AGAGACTTCCTTGATTCGACCGTGACTGGAGTTCAGACGTGTGCTCATCA370.14036951325922833TruSeq Adapter, Index 11 (96% over 30bp)
AGAGATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTGA360.13657574263060054No Hit
AGAGACTTCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTGA340.12898820137334496No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGGG310.11760688948746159No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGCG290.110019348230206No Hit
AGAGAATTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG280.1062255776015782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACT25950.0125.01
AGACTTC26050.0124.7600863
GAGACTT26050.0124.7600862
GACTTCC26050.0124.5201644
ACTTCCT26000.0124.519225
TCCTTGA26100.0124.281618
CCTTGAT26100.0124.281619
TTCCTTG26100.0124.281617
CTTCCTT26050.0124.2802356
TAGAAGA150.00346815162.574-75
AAGGAAA150.00346815162.578-79
GAAAGAA150.00346815162.580-81
GTATGCT7500.062.49999636-37
ATGCTTT7500.062.49999638-39
GCTTTAG7500.062.49999640-41
TTGATTC26150.062.38049710-11
GATTCGA25950.062.37957812-13
GATGGGC25900.062.3793560-61
TTGTCTA25900.062.3793524-25
TTCGACC25900.062.3793514-15
Produced by FastQC (version 0.11.5)