FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_NSFL1C_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_NSFL1C_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7598
Sequences flagged as poor quality0
Sequence length131
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA369048.565411950513294No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA175523.0981837325612No Hit
GTCTGCAAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA76610.081600421163465Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA1882.4743353514082655No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA1712.250592261121348No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA1241.6320084232692815No Hit
GTCTGCCAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA480.631745196104238Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCAAGCCGAATTTGGATGTTTGTGGCAGGCTCTGATTCGTTGATTA270.3553566728086338No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA250.3290339563042906No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA170.2237430902869176No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGAAGGCTCTGATTCGTTGATTA100.13161358252171623No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTAA100.13161358252171623No Hit
GTCTGCAAGCCGAATTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTAG100.13161358252171623Illumina Single End PCR Primer 1 (100% over 22bp)
GTCTGCAAGCCGAATTTGGAGTGACTGGAGTTCAGACGTGTGCTCTTCAT100.13161358252171623TruSeq Adapter, Index 6 (96% over 33bp)
GTTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA90.11845222426954462No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTCGGCTCTGATTCGTTGATTA90.11845222426954462No Hit
GTCTGCAAGCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA80.10529086601737299No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCG353.092282E-11125.000016
GTCTGCC353.092282E-11125.000011
GCCAGCC353.092282E-11125.000015
TCTGCCA353.092282E-11125.000012
TGCCAGC353.092282E-11125.000014
CTGCCAG353.092282E-11125.000013
CAGCCGA353.092282E-11125.000017
TCTGCAA7050.0125.02
CTGCAAG7000.0125.03
TGCAAGC7100.0124.119734
GTCTGCA7100.0124.119731
AGCCGAA7450.0123.322148
GCCGAAT7450.0123.322149
GCAAGCC7150.0123.2517555
AAGCCGA7150.0123.2517557
CAAGCCG7150.0123.2517556
CGACAAT358.3164196E-962.50000442-43
TCGACAA358.3164196E-962.50000440-41
TGGTAGT358.3164196E-962.50000426-27
TGGTAGG5750.062.50000426-27
Produced by FastQC (version 0.11.5)