FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_NSFL1C_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_NSFL1C_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA372052.64647608264931No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA179525.403339937729974No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA4486.3402207755448625No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA2233.1559581092555904No Hit
GTCTGCAAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA1782.5191055759977354Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA1011.4293801302009623No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA290.41041607698839516No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA260.3679592414378715No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTAA200.2830455703368242No Hit
GTCTGCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA120.16982734220209456No Hit
GTCTGCAAGCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA90.1273705066515709No Hit
GTCTGCAAGCTGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA90.1273705066515709No Hit
GTCTGCAAGCCGATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA80.11321822813472968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGCC6000.0125.05
TGCAAGC6000.0125.04
GTCTGCA6050.0125.01
TCTGCAA6000.0125.02
CTGCAAG6000.0125.03
CAAGCCG6050.0125.06
CCAGCCG700.0124.999996
GTCTGCC700.0124.999991
GCCAGCC700.0124.999995
TCTGCCA700.0124.999992
TGCCAGC700.0124.999994
CTGCCAG700.0124.999993
AAGCCGA6100.0123.975427
AGCCGAA6850.0123.1751868
GCCGAAT6850.0123.1751869
CAGCCGA750.0116.666677
TTCATTT6850.062.50000472-73
TGGTAGG6250.062.50000426-27
GCTGGCT6850.062.50000460-61
TGATTCG6850.062.50000436-37
Produced by FastQC (version 0.11.5)