FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_NSFL1C_G2mut.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_NSFL1C_G2mut.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22920
Sequences flagged as poor quality0
Sequence length131
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA1125249.09249563699826No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA603326.321989528795815No Hit
GTCTGCAAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA13996.103839441535777Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA7373.2155322862129148No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA6792.962478184991274No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA3571.557591623036649No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA770.33595113438045376No Hit
GTCTGCCAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA620.27050610820244325Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA450.1963350785340314No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTAA430.18760907504363No Hit
GTCTGCAAGCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA360.15706806282722513No Hit
GTCTGCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA290.12652705061082023No Hit
GTCTGCAAGCCGATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA260.11343804537521816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCG1350.0125.06
GTCTGCC1300.0125.01
GTCTGCA20750.0125.01
GCCAGCC1350.0125.05
TCTGCCA1350.0125.02
TGCCAGC1350.0125.04
CTGCCAG1350.0125.03
CAGCCGA1300.0125.07
TCTGCAA20850.0124.700242
CTGCAAG20800.0124.699523
GCAAGCC20700.0124.6980745
TGCAAGC20800.0124.399034
CAAGCCG20800.0124.399036
AGCCGAA22000.0124.147738
AAGCCGA20850.0124.100727
GCCGAAT22000.0123.863649
GGTGACT150.00346524662.50000418-19
GAGTGTA150.00346524662.500004118-119
GTCGATT7100.062.50000442-43
ATTCGTC7100.062.50000438-39
Produced by FastQC (version 0.11.5)