FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_NSFL1C_G1wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_NSFL1C_G1wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19557
Sequences flagged as poor quality0
Sequence length131
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA1064354.42041212864959No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA370518.944623408498234No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA12056.161476709106713No Hit
GTCTGCAAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA8794.494554379506059Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA6833.492355678273764No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTA1940.9919721838727821No Hit
GTCTGCCAGCCGAATTTGGATGTTTGTGGTAGTCTCTGATTCGACAATTA690.352814848903206No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA600.3067955207853965No Hit
GTCTGCCAGCCGAATTTTCTGCCTGTTGGAGATCGGAAGAGCGTCGTGTA500.2556629339878304Illumina Single End PCR Primer 1 (100% over 21bp)
GTCTGCAAGCCGAATTTGGAGTGACTGGAGTTCAGACGTGTGCTTCATTT430.21987012322953417TruSeq Adapter, Index 6 (96% over 29bp)
GTCTGCAAGCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA310.15851101907245488No Hit
GTCTGCAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA280.14317124303318507No Hit
GTCTGAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTCGATTAA270.13805798435342845No Hit
GTCTGCAAGCCGAATTTGGAAGAGCGTCGTGTAGGGAAAGACTTCATTTT270.13805798435342845Illumina Single End PCR Primer 1 (96% over 26bp)
GTTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA270.13805798435342845No Hit
GTCTGCAAGCCGAATTTAGATGTTTGTGGTAGGCTCTGATTCGTTGATTA210.10737843227488879No Hit
GTCTGCAAGCCGATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTAA200.10226517359513218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCG1300.0125.000016
GTCTGCC1300.0125.000011
GCCAGCC1300.0125.000015
TCTGCCA1300.0125.000012
TGCCAGC1300.0125.000014
CTGCCAG1300.0125.000013
GTCTGCA17000.0125.01
GAAGCCG204.3492E-6124.999995
TGAAGCC204.3492E-6124.999994
AAGCCAA152.1883627E-4124.999997
CAAGCCA152.1883627E-4124.999996
TCTGCAA16950.0124.631272
CTGCAAG16900.0124.630183
TGCAAGC16900.0124.260354
GCAAGCC16900.0123.890535
CAAGCCG16900.0123.520716
AAGCCGA17200.0122.093027
GCCGAAT18450.0121.9512259
AGCCGAA18450.0121.612478
CAGCCGA1400.0116.071437
Produced by FastQC (version 0.11.5)