FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_IMMT_WT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_IMMT_WT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30675
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA2960296.50203748981255No Hit
TGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG460.14995925020374898No Hit
CTGTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG450.1466992665036675No Hit
CTGTTGCTGAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG430.1401792991035045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCT30150.0125.02
CTGTTGC30100.0125.01
GTTGCTG30200.0124.793053
TTGCTGA30350.0124.176284
TGCTGAA30300.0124.174925
CTGAAGC30300.0124.174927
GCTGAAG30300.0124.174926
GAAGCTG30350.0123.9703459
TGAAGCT30350.0123.9703458
GTGTTGT150.003470857262.538-39
ATGGACC150.003470857262.596-97
GACTGTT10650.062.550-51
CAGACTG10650.062.548-49
GGCAGAC10650.062.546-47
TAGAGAT30500.062.29508284-85
ACTAGAG30500.062.29508282-83
GAGATTT30500.062.29508286-87
TTGTTTT30450.062.29474328-29
ACACTAG30450.062.29474380-81
TGATACA30450.062.29474376-77
Produced by FastQC (version 0.11.5)