FastQCFastQC Report
Fri 27 Sep 2019
000000000-D798V_l01_n02_ms_IMMT_NT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-D798V_l01_n02_ms_IMMT_NT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10100
Sequences flagged as poor quality0
Sequence length131
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA974196.44554455445544No Hit
TGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG150.1485148514851485No Hit
CTGTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG140.1386138613861386No Hit
CTTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCAG130.12871287128712872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCT9850.0125.02
CTGTTGC9800.0125.01
GTTGCTG9850.0124.3654863
GAAGCTG10000.0123.1259
TGAAGCT10000.0123.1258
TGCTGAA9950.0123.1155855
CTGAAGC9950.0123.1155857
TTGCTGA9950.0123.1155854
GCTGAAG10000.0122.56
GAGGCAG9950.062.50000444-45
GTGAGGC9950.062.50000442-43
TCTGATA9950.062.50000474-75
GACTGTT3600.062.50000450-51
ATTCTTT9950.062.50000460-61
CTTCTGA9950.062.50000472-73
TGATACA10000.062.576-77
GTTTAGA9900.062.554-55
TCTTTCA9850.062.562-63
TTGTGAG10000.062.540-41
AGATTCT9850.062.558-59
Produced by FastQC (version 0.11.5)